Relaxation of Bovine Mesenteric Artery Induced by Glyceryl Trinitrate is Attenuated by Pertussis Toxin

Johan Ahlner, Krister L. Axelsson, Marie Ekstram‐Ljusegren, Richard L. Friedman, Nils Gnindström, Ja Olof G. Karlsson, Rolf G.G. Andersson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Abstract: Low concentrations (< 1 nM) of glyceryl trinitrate (GTN) induced a considerable relaxation of bovine mesenteric arteries (BMA) brought to sustained contraction by the addition of phenylephrine. The concentration‐response curve of GTN showed a biphasic pattern with a high and a low affinity component. Pretreatment with pertussis toxin (IAP) attenuated the high affinity relaxant component, but not the low affinity component or the relaxation induced by NaNO2. A polyclonal antibody to IAP counteracted the effect of the toxin on the GTN‐response. Low concentrations of GTN increased the cGMP level in BMA via activation of the high affinity pathway. This effect was also inhibited in preparations pretreated with IAP. It is suggested from the present study that GTN induces relaxation of vascular smooth muscle via two separate pathways, the high affinity pathway might involve a receptor‐complex interaction with a regulatory protein. 1988 Nordic Pharmacological Society

Original languageEnglish (US)
Pages (from-to)155-158
Number of pages4
JournalPharmacology & Toxicology
Volume62
Issue number3
DOIs
StatePublished - Mar 1988

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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    Ahlner, J., Axelsson, K. L., Ekstram‐Ljusegren, M., Friedman, R. L., Gnindström, N., Karlsson, J. O. G., & Andersson, R. G. G. (1988). Relaxation of Bovine Mesenteric Artery Induced by Glyceryl Trinitrate is Attenuated by Pertussis Toxin. Pharmacology & Toxicology, 62(3), 155-158. https://doi.org/10.1111/j.1600-0773.1988.tb01864.x