Relaxation of bovine mesenteric artery induced by glyceryl trinitrate is attenuated by pertussis toxin

J. Ahlner, K. L. Axelsson, M. Ekstram-Ljusegren, Richard L Friedman, N. Grundstrom, J. O G Karlsson, R. G G Andersson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Low concentrations (< 1 nM) of glyceryl trinitrate (GTN) induced a considerable relaxation of bovine mesenteric arteries (BMA) brought to sustained contraction by the addition of phenylephrine. The concentration-response curve of GTN showed a biphasic pattern with a high and a low affinity component. Pretreatment with pertussis toxin (IAP) attenuated the high affinity relaxant component, but not the low affinity component or the relaxation induced by NaNO2. A polyclonal antibody to IAP counteracted the effect of the toxin on the GTN-response. Low concentrations of GTN increased to cGMP level in BMA via activation of the high affinity pathway. This effect was also inhibited in preparations pretreated with IAP. It is suggested from the present study that GTN induced relaxation of vascular smooth muscle via two separate pathways, the high affinity pathway might involve a receptor-complex interaction with a regulatory protein.

Original languageEnglish (US)
Pages (from-to)155-158
Number of pages4
JournalPharmacology and Toxicology
Volume62
Issue number3
StatePublished - 1988

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Mesenteric Arteries
Pertussis Toxin
Nitroglycerin
Phenylephrine
Vascular Smooth Muscle
Muscle
Chemical activation
Antibodies
Proteins

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Pharmacology
  • Toxicology

Cite this

Ahlner, J., Axelsson, K. L., Ekstram-Ljusegren, M., Friedman, R. L., Grundstrom, N., Karlsson, J. O. G., & Andersson, R. G. G. (1988). Relaxation of bovine mesenteric artery induced by glyceryl trinitrate is attenuated by pertussis toxin. Pharmacology and Toxicology, 62(3), 155-158.

Relaxation of bovine mesenteric artery induced by glyceryl trinitrate is attenuated by pertussis toxin. / Ahlner, J.; Axelsson, K. L.; Ekstram-Ljusegren, M.; Friedman, Richard L; Grundstrom, N.; Karlsson, J. O G; Andersson, R. G G.

In: Pharmacology and Toxicology, Vol. 62, No. 3, 1988, p. 155-158.

Research output: Contribution to journalArticle

Ahlner, J, Axelsson, KL, Ekstram-Ljusegren, M, Friedman, RL, Grundstrom, N, Karlsson, JOG & Andersson, RGG 1988, 'Relaxation of bovine mesenteric artery induced by glyceryl trinitrate is attenuated by pertussis toxin', Pharmacology and Toxicology, vol. 62, no. 3, pp. 155-158.
Ahlner J, Axelsson KL, Ekstram-Ljusegren M, Friedman RL, Grundstrom N, Karlsson JOG et al. Relaxation of bovine mesenteric artery induced by glyceryl trinitrate is attenuated by pertussis toxin. Pharmacology and Toxicology. 1988;62(3):155-158.
Ahlner, J. ; Axelsson, K. L. ; Ekstram-Ljusegren, M. ; Friedman, Richard L ; Grundstrom, N. ; Karlsson, J. O G ; Andersson, R. G G. / Relaxation of bovine mesenteric artery induced by glyceryl trinitrate is attenuated by pertussis toxin. In: Pharmacology and Toxicology. 1988 ; Vol. 62, No. 3. pp. 155-158.
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