Release of hepatocyte growth factor from mechanically stretched skeletal muscle satellite cells and role of pH and nitric oxide

Ryuichi Tatsumi, Akihito Hattori, Yoshihide Ikeuchi, Judy E. Anderson, Ronald E Allen

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

Application of mechanical stretch to cultured adult rat muscle satellite cells results in release of hepatocyte growth factor (HGF) and accelerated entry into the cell cycle. Stretch activation of cultured rat muscle satellite cells was observed only when medium pH was between 7.1 and 7.5, even though activation of satellite cells was accelerated by exogenous HGF over a pH range from 6.9 to 7.8. Furthermore, HGF was only released in stretched cultures when the pH of the medium was between 7.1 and 7.4. Conditioned medium from stretched satellite cell cultures stimulated activation of unstretched satellite cells, and the addition of anti-HGF neutralizing antibodies to stretch-conditioned medium inhibited the stretch activation response. Conditioned medium from satellite cells that were stretched in the presence of nitric-oxide synthase (NOS) inhibitor Nω-nitro-L-arginine methyl ester hydrochloride did not accelerate activation of unstretched control satellite cells, and HGF was not released into the medium. Conditioned medium from unstretched cells that were treated with a nitric oxide donor, sodium nitroprusside dihydrate, was able to accelerate the activation of satellite cells in vitro, and HGF was found in the conditioned medium. Immunoblot analysis indicated that both neuronal and endothelial NOS isoforms were present in satellite cell cultures. Furthermore, assays of NOS activity in stretched satellite cell cultures demonstrated that NOS is stimulated when satellite cells are stretched in vitro. These experiments indicate that stretch triggers an intracellular cascade of events, including nitric oxide synthesis, which results in HGF release and satellite cell activation.

Original languageEnglish (US)
Pages (from-to)2909-2918
Number of pages10
JournalMolecular Biology of the Cell
Volume13
Issue number8
DOIs
StatePublished - Aug 2002

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Skeletal Muscle Satellite Cells
Hepatocyte Growth Factor
Nitric Oxide
Conditioned Culture Medium
Nitric Oxide Synthase
Cell Culture Techniques
Muscle Cells
Nitric Oxide Synthase Type I
Nitric Oxide Donors
Nitric Oxide Synthase Type III
Nitroprusside
Neutralizing Antibodies
Arginine
Cell Cycle
Protein Isoforms

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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Release of hepatocyte growth factor from mechanically stretched skeletal muscle satellite cells and role of pH and nitric oxide. / Tatsumi, Ryuichi; Hattori, Akihito; Ikeuchi, Yoshihide; Anderson, Judy E.; Allen, Ronald E.

In: Molecular Biology of the Cell, Vol. 13, No. 8, 08.2002, p. 2909-2918.

Research output: Contribution to journalArticle

Tatsumi, Ryuichi ; Hattori, Akihito ; Ikeuchi, Yoshihide ; Anderson, Judy E. ; Allen, Ronald E. / Release of hepatocyte growth factor from mechanically stretched skeletal muscle satellite cells and role of pH and nitric oxide. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 8. pp. 2909-2918.
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