Renal tubular reabsorption of taurine, γ-aminobutyric acid (GABA) and β-alanine studied by continuous microperfusion

W. H. Dantzler, S. Silbernagl

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Renal tubular reabsorption of taurine, γ-aminobutyric acid (GABA), and β-alanine was studied in vivo et situ by continuous microperfusion of single proximal tubules of the rat. In each case, reabsorption was much slower than that for other amino acids that have been studied. With a concentration of 0.1 mmol/l in initial perfusate, about 60% of initial load was reabsorbed over perfusion distance of 3 mm. Taurine reabsorption saturated with only 2.17 mmol/l in initial perfusate. Assuming simple two-parameter kinetics, upper limits for Km of 0.54 mmol/l and for Vmax of 0.59 pmol·cm-1·s-1 for tubular reabsorption of taurine were estimated. High (20 mmol/l) concentrations of taurine or β-alanine in perfusate completely inhibited GABA reabsorption, but l-phenylalanine (20 mmol/l) had no significant effect. The results indicate that the three amino acids are reabsorbed slowly from the proximal tubule by what may be a common transport system. This system appears to have a high affinity but low capacity and to be different from other known renal tubular transport systems for amino acids.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalPflügers Archiv European Journal of Physiology
Volume367
Issue number2
DOIs
StatePublished - Jan 1 1976

Keywords

  • Amino acid transport
  • Renal tubule
  • Taurine
  • β-Alanine
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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