Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T

Thomas E. Yankeelov, Laura M. DeBusk, David D Billheimer, Jeffrey J. Luci, P. Charles Lin, Ronald R. Price, John C. Gore

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Purpose: To test the repeatability of a reference region (RR) model for the analysis of dynamic contrast-enhanced MRI (DCE-MRI) in a mouse model of cancer at high field. Materials and Methods: Seven mice were injected with 10 6 4T1 mammary carcinoma cells and imaged eight to 10 days later on a Varian 7.0T scanner. Two DCE-MRI studies were performed for each mouse (separated by 2.5 hours). The RR model was used to analyze the data, and returned estimates on the perfusion-permeability index (Ktrans) for the RR and the tissue of interest (TOI), as well as the extravascular extracellular volume fraction (ve) for the TOI. Results: When the first injection was compared with the second injection, all parameters tested were highly correlated (r2 = 0.90, 0.62, 0.82 for the RR K trans, TOI Ktrans, and TOI ve, respectively, with P < 0.001 for all). To observe a statistically significant change (at the 5% level) in a treatment study with seven animals in each group, log 10 changes of 0.084 and 0.077 in the tumor Ktrans and ve. respectively, are required. Conclusion: If a reliable arterial input function (AIF) is unavailable, the RR model is a reasonable alternative to measuring MRI contrast-agent (CA) kinetics in mouse models of cancer at high field.

Original languageEnglish (US)
Pages (from-to)1140-1147
Number of pages8
JournalJournal of Magnetic Resonance Imaging
Volume24
Issue number5
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Neoplasms
Injections
Contrast Media
Permeability
Perfusion
Breast Neoplasms
Therapeutics

Keywords

  • DCE-MRI
  • Kinetic modelling
  • Reference region
  • Repeatability
  • Tumor pharmacokinetics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Yankeelov, T. E., DeBusk, L. M., Billheimer, D. D., Luci, J. J., Lin, P. C., Price, R. R., & Gore, J. C. (2006). Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T. Journal of Magnetic Resonance Imaging, 24(5), 1140-1147. https://doi.org/10.1002/jmri.20729

Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T. / Yankeelov, Thomas E.; DeBusk, Laura M.; Billheimer, David D; Luci, Jeffrey J.; Lin, P. Charles; Price, Ronald R.; Gore, John C.

In: Journal of Magnetic Resonance Imaging, Vol. 24, No. 5, 11.2006, p. 1140-1147.

Research output: Contribution to journalArticle

Yankeelov, Thomas E. ; DeBusk, Laura M. ; Billheimer, David D ; Luci, Jeffrey J. ; Lin, P. Charles ; Price, Ronald R. ; Gore, John C. / Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T. In: Journal of Magnetic Resonance Imaging. 2006 ; Vol. 24, No. 5. pp. 1140-1147.
@article{5809c589b99a432d9c8abc84256d0ed6,
title = "Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T",
abstract = "Purpose: To test the repeatability of a reference region (RR) model for the analysis of dynamic contrast-enhanced MRI (DCE-MRI) in a mouse model of cancer at high field. Materials and Methods: Seven mice were injected with 10 6 4T1 mammary carcinoma cells and imaged eight to 10 days later on a Varian 7.0T scanner. Two DCE-MRI studies were performed for each mouse (separated by 2.5 hours). The RR model was used to analyze the data, and returned estimates on the perfusion-permeability index (Ktrans) for the RR and the tissue of interest (TOI), as well as the extravascular extracellular volume fraction (ve) for the TOI. Results: When the first injection was compared with the second injection, all parameters tested were highly correlated (r2 = 0.90, 0.62, 0.82 for the RR K trans, TOI Ktrans, and TOI ve, respectively, with P < 0.001 for all). To observe a statistically significant change (at the 5{\%} level) in a treatment study with seven animals in each group, log 10 changes of 0.084 and 0.077 in the tumor Ktrans and ve. respectively, are required. Conclusion: If a reliable arterial input function (AIF) is unavailable, the RR model is a reasonable alternative to measuring MRI contrast-agent (CA) kinetics in mouse models of cancer at high field.",
keywords = "DCE-MRI, Kinetic modelling, Reference region, Repeatability, Tumor pharmacokinetics",
author = "Yankeelov, {Thomas E.} and DeBusk, {Laura M.} and Billheimer, {David D} and Luci, {Jeffrey J.} and Lin, {P. Charles} and Price, {Ronald R.} and Gore, {John C.}",
year = "2006",
month = "11",
doi = "10.1002/jmri.20729",
language = "English (US)",
volume = "24",
pages = "1140--1147",
journal = "Journal of Magnetic Resonance Imaging",
issn = "1053-1807",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Repeatability of a reference region model for analysis of murine DCE-MRI data at 7T

AU - Yankeelov, Thomas E.

AU - DeBusk, Laura M.

AU - Billheimer, David D

AU - Luci, Jeffrey J.

AU - Lin, P. Charles

AU - Price, Ronald R.

AU - Gore, John C.

PY - 2006/11

Y1 - 2006/11

N2 - Purpose: To test the repeatability of a reference region (RR) model for the analysis of dynamic contrast-enhanced MRI (DCE-MRI) in a mouse model of cancer at high field. Materials and Methods: Seven mice were injected with 10 6 4T1 mammary carcinoma cells and imaged eight to 10 days later on a Varian 7.0T scanner. Two DCE-MRI studies were performed for each mouse (separated by 2.5 hours). The RR model was used to analyze the data, and returned estimates on the perfusion-permeability index (Ktrans) for the RR and the tissue of interest (TOI), as well as the extravascular extracellular volume fraction (ve) for the TOI. Results: When the first injection was compared with the second injection, all parameters tested were highly correlated (r2 = 0.90, 0.62, 0.82 for the RR K trans, TOI Ktrans, and TOI ve, respectively, with P < 0.001 for all). To observe a statistically significant change (at the 5% level) in a treatment study with seven animals in each group, log 10 changes of 0.084 and 0.077 in the tumor Ktrans and ve. respectively, are required. Conclusion: If a reliable arterial input function (AIF) is unavailable, the RR model is a reasonable alternative to measuring MRI contrast-agent (CA) kinetics in mouse models of cancer at high field.

AB - Purpose: To test the repeatability of a reference region (RR) model for the analysis of dynamic contrast-enhanced MRI (DCE-MRI) in a mouse model of cancer at high field. Materials and Methods: Seven mice were injected with 10 6 4T1 mammary carcinoma cells and imaged eight to 10 days later on a Varian 7.0T scanner. Two DCE-MRI studies were performed for each mouse (separated by 2.5 hours). The RR model was used to analyze the data, and returned estimates on the perfusion-permeability index (Ktrans) for the RR and the tissue of interest (TOI), as well as the extravascular extracellular volume fraction (ve) for the TOI. Results: When the first injection was compared with the second injection, all parameters tested were highly correlated (r2 = 0.90, 0.62, 0.82 for the RR K trans, TOI Ktrans, and TOI ve, respectively, with P < 0.001 for all). To observe a statistically significant change (at the 5% level) in a treatment study with seven animals in each group, log 10 changes of 0.084 and 0.077 in the tumor Ktrans and ve. respectively, are required. Conclusion: If a reliable arterial input function (AIF) is unavailable, the RR model is a reasonable alternative to measuring MRI contrast-agent (CA) kinetics in mouse models of cancer at high field.

KW - DCE-MRI

KW - Kinetic modelling

KW - Reference region

KW - Repeatability

KW - Tumor pharmacokinetics

UR - http://www.scopus.com/inward/record.url?scp=33750432502&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750432502&partnerID=8YFLogxK

U2 - 10.1002/jmri.20729

DO - 10.1002/jmri.20729

M3 - Article

C2 - 17024660

AN - SCOPUS:33750432502

VL - 24

SP - 1140

EP - 1147

JO - Journal of Magnetic Resonance Imaging

JF - Journal of Magnetic Resonance Imaging

SN - 1053-1807

IS - 5

ER -