Repression of Manduca sexta ferritin synthesis by IRP1/ire interaction

D. Zhang, D. W. Albert, P. Kohlhepp, D. Q. D.-Pham, Joy Winzerling

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mammalian ferritin subunit synthesis is controlled at the translational level by the iron regulatory protein 1 (IRP1)/iron responsive element (IRE) interaction. Insect haemolymph ferritin subunit messages have an IRE in the 5′-untranslated region (UTR). We have shown that recombinant M. sexta IRP1 represses the in vitro translation of both the heavy and light chain ferritin subunits from this species without altering transcription. Deletion of either the 5′-UTR or the IRE from the mRNA abolishes IRP1 repression. Our studies indicated that the translational control of ferritin synthesis by IRP/IRE interaction could occur in insects in a manner similar to that of mammals. To our knowledge, this is the first report of the control of insect ferritin synthesis by IRP1/IRE interaction. Furthermore, this is the first indication that the synthesis of a secreted ferritin subunit can also be controlled in this manner.

Original languageEnglish (US)
Pages (from-to)531-539
Number of pages9
JournalInsect Molecular Biology
Volume10
Issue number6
DOIs
StatePublished - 2001

Fingerprint

Iron Regulatory Protein 1
Manduca
Manduca sexta
regulatory proteins
ferritin
Ferritins
Iron
iron
synthesis
Apoferritins
5' Untranslated Regions
Insects
Insect Control
5' untranslated regions
Mammals
Hemolymph
Transcription
insects
insect control
Messenger RNA

Keywords

  • Ferritin synthesis
  • IRE
  • Iron
  • IRP1
  • Manduca sexta
  • Translational control

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Repression of Manduca sexta ferritin synthesis by IRP1/ire interaction. / Zhang, D.; Albert, D. W.; Kohlhepp, P.; D.-Pham, D. Q.; Winzerling, Joy.

In: Insect Molecular Biology, Vol. 10, No. 6, 2001, p. 531-539.

Research output: Contribution to journalArticle

Zhang, D. ; Albert, D. W. ; Kohlhepp, P. ; D.-Pham, D. Q. ; Winzerling, Joy. / Repression of Manduca sexta ferritin synthesis by IRP1/ire interaction. In: Insect Molecular Biology. 2001 ; Vol. 10, No. 6. pp. 531-539.
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