Requirement of pointed-end capping by tropomodulin to maintain actin filament length in embryonic chick cardiac myocytes

Carol Gregorio, Annemarie Weber, Meredith Bondad, Cynthia R. Pennise, Velia M. Fowler

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Control of actin filament length and dynamics is important for cell motility and architecture and is regulated in part by capping proteins that block elongation and depolymerization at both the fast-growing (barbed) and slow-growing (pointed) ends. Tropomodulin is a capping protein for the pointed end of the actin filament; it is associated with the free, pointed ends of the thin filaments in striated muscle, where it is thought to bind to both tropomyosin and actin. In embryonic chick cardiac myocytes, tropomodulin assembles after the thin, as well as the thick, filaments have become organized into periodic I and A bands, suggesting that tropomodulin might be involved in maintaining actin filament length. Here we show that microinjection of an antibody that inhibits tropomodulin's pointed-end-capping activity in vitro results in a marked elongation of actin filaments from their pointed ends and a > 80% reduction in the percentage of beating cells. This demonstrates that pointed-end capping by tropomodulin is required to maintain actin filament length in vivo and that this is essential for contractile function in embryonic chick cardiac myocytes.

Original languageEnglish (US)
Pages (from-to)83-86
Number of pages4
JournalNature
Volume377
Issue number6544
DOIs
StatePublished - Sep 7 1995
Externally publishedYes

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Tropomodulin
Actin Cytoskeleton
Cardiac Myocytes
Tropomyosin
Striated Muscle
Microinjections
Cell Movement
Actins
Proteins
Antibodies

ASJC Scopus subject areas

  • General

Cite this

Requirement of pointed-end capping by tropomodulin to maintain actin filament length in embryonic chick cardiac myocytes. / Gregorio, Carol; Weber, Annemarie; Bondad, Meredith; Pennise, Cynthia R.; Fowler, Velia M.

In: Nature, Vol. 377, No. 6544, 07.09.1995, p. 83-86.

Research output: Contribution to journalArticle

Gregorio, Carol ; Weber, Annemarie ; Bondad, Meredith ; Pennise, Cynthia R. ; Fowler, Velia M. / Requirement of pointed-end capping by tropomodulin to maintain actin filament length in embryonic chick cardiac myocytes. In: Nature. 1995 ; Vol. 377, No. 6544. pp. 83-86.
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