Resistance to Bacillus thuringiensis toxin Cry2Ab and survival on single-toxin and pyramided cotton in cotton bollworm from China

Laipan Liu, Meijing Gao, Song Yang, Shaoyan Liu, Yidong Wu, Yves Carriere, Yihua Yang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Evolution of Helicoverpa armigera resistance to Bacillus thuringiensis (Bt) cotton producing Cry1Ac is progressing in northern China, and replacement of Cry1Ac cotton by pyramided Bt cotton has been considered to counter such resistance. Here, we investigated four of the eight conditions underlying success of the refuge strategy for delaying resistance to Cry1Ac+Cry2Ab cotton, a pyramid that has been used extensively against H. armigera outside China. Laboratory bioassays of a Cry2Ab-selected strain (An2Ab) and a related unselected strain (An) reveal that resistance to Cry2Ab (130-fold) was nearly dominant, autosomally inherited, and controlled by more than one locus. Strong cross-resistance occurred between Cry2Ab and Cry2Aa (81-fold). Weaker cross-resistance (18- to 22-fold) between Cry2Ab and Cry1A toxins was also present and significantly increased survival of An2Ab relative to An on cotton cultivars producing the fusion protein Cry1Ac/Cry1Ab or Cry1Ac. Survival on Cry1Ac+Cry2Ab cotton was also significantly higher in An2Ab than in An, showing that redundant killing on this pyramid was incomplete. Survival on non-Bt cotton did not differ significantly between An2Ab and An, indicating an absence of fitness costs affecting this trait. These results indicate that a switch to three-toxin pyramided cotton could be valuable for increasing durability of Bt cotton in China.

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalEvolutionary Applications
Volume10
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Bacillus thuringiensis
Helicoverpa armigera
toxin
cotton
China
toxins
Biological Assay
cross resistance
fold
Costs and Cost Analysis
Proteins
durability
refuge
bioassay
cultivar
fitness
bioassays
replacement
loci
protein

Keywords

  • Bt cotton
  • cross-resistance
  • dominance of resistance
  • fitness costs
  • pyramided crops
  • redundant killing
  • resistance management

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Agricultural and Biological Sciences(all)

Cite this

Resistance to Bacillus thuringiensis toxin Cry2Ab and survival on single-toxin and pyramided cotton in cotton bollworm from China. / Liu, Laipan; Gao, Meijing; Yang, Song; Liu, Shaoyan; Wu, Yidong; Carriere, Yves; Yang, Yihua.

In: Evolutionary Applications, Vol. 10, No. 2, 01.02.2017, p. 170-179.

Research output: Contribution to journalArticle

Liu, Laipan ; Gao, Meijing ; Yang, Song ; Liu, Shaoyan ; Wu, Yidong ; Carriere, Yves ; Yang, Yihua. / Resistance to Bacillus thuringiensis toxin Cry2Ab and survival on single-toxin and pyramided cotton in cotton bollworm from China. In: Evolutionary Applications. 2017 ; Vol. 10, No. 2. pp. 170-179.
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