Resolution of complex neuronal arrangements in the blowfly visual system using triple fluorescence staining

N. J. Strausfeld, H. S. Seyan

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Consecutive application of the fluorescent dyes, Texas Red and Lucifer Yellow, followed by nuclear staining with Bisbenzimide, reveals spatial relationships between individual neurons and relationships between their cell bodies and the perikaryal rind. The method is particularly useful for light-microscopical studies of complex interrelationships between identified neurons. The method has specific advantages over intraneuropil staining with cobalt or with HRP. These advantages are: simplicity, speed, information content, and aesthetic considerations.

Original languageEnglish (US)
Pages (from-to)5-10
Number of pages6
JournalCell and Tissue Research
Volume247
Issue number1
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Keywords

  • Blowfly, Calliphora erythrocephala
  • Central nervous system
  • Fluorescent dyes
  • Neuronal arrangements

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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