Resolution of intraneuronal and transynaptic migration of cobalt in the insect visual and central nervous systems

Nicholas J Strausfeld, M. Obermayer

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Conventional cobalt chloride procedures, using either diffusion into cut nerves or application via an electrode, can only incompletely resolve nerve cells for light microscopical observation. Enhanced resolution can be obtained by the addition of small quantities of serum albumin to the cobalt chloride. This allows sufficient contrast many nerve cells and enhances the details of thin fibres and their appendages. That this should be possible is of cardinal importance for combined electrophysiological and structural studies. The present account also illustrates that in Dipterous insects there are certain conditions where cobalt chloride will move from one neuron into another. This movement is between functionally contiguous neurons and is here described from the visual system, with reference to electrophysiological studies that employ procion yellow identifications. Transynaptically filled neurons are only faintly resoluble in whole mounts. A block modification of the Timm's enhancement procedure is described which will reveal the finest details of neurons in thick sections and will also reveal nerve cells that have taken up minute quantities of CoCl2 after passage across at least two synapses. These observations are discussed with reference to their implications and applications for electrophysiological mappings of structural pathways.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalJournal of Comparative Physiology A
Volume110
Issue number1
DOIs
StatePublished - Jan 1976
Externally publishedYes

Fingerprint

nervous system
cobalt
Cobalt
central nervous system
Insects
Central Nervous System
neurons
chloride
insect
Neurons
insects
chlorides
serum
electrode
serum albumin
synapse
appendages
Serum Albumin
Synapses
electrodes

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Neuroscience(all)
  • Physiology (medical)
  • Physiology
  • Animal Science and Zoology

Cite this

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abstract = "Conventional cobalt chloride procedures, using either diffusion into cut nerves or application via an electrode, can only incompletely resolve nerve cells for light microscopical observation. Enhanced resolution can be obtained by the addition of small quantities of serum albumin to the cobalt chloride. This allows sufficient contrast many nerve cells and enhances the details of thin fibres and their appendages. That this should be possible is of cardinal importance for combined electrophysiological and structural studies. The present account also illustrates that in Dipterous insects there are certain conditions where cobalt chloride will move from one neuron into another. This movement is between functionally contiguous neurons and is here described from the visual system, with reference to electrophysiological studies that employ procion yellow identifications. Transynaptically filled neurons are only faintly resoluble in whole mounts. A block modification of the Timm's enhancement procedure is described which will reveal the finest details of neurons in thick sections and will also reveal nerve cells that have taken up minute quantities of CoCl2 after passage across at least two synapses. These observations are discussed with reference to their implications and applications for electrophysiological mappings of structural pathways.",
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