Response characteristics of an identified, sexually dimorphic olfactory glomerulus

Jane Roche King, Thomas A. Christensen, John G Hildebrand

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Partitioning of synaptic neuropil into glomeruli is a common feature of primary olfactory centers in most animal species. The functional significance of glomeruli, however, is not yet well understood. The present study is part of our effort to test the hypothesis that each glomerulus is a functional unit dedicated to processing information about a particular odorant or attribute of odor molecules and that the glomerular array constitutes a map of 'odor space.' We investigated the physiological and morphological features of uniglomerular projection neurons (PNs) associated with an identified glomerulus in each antennal lobe of the female sphinx moth, Manduca sexta. This 'lateral large female glomerulus' (latLFG) is sexually dimorphic and therefore may play a female-specific role, such as processing of information about one or more odorants important for orientation of a female to host plants for oviposition. Together with the medial LFG (medLFG), the latLFG resides outside the array of spheroidal ordinary glomeruli, near the entrance of the antennal (olfactory) nerve. Each LFG is innervated by four to five PNs. Using intracellular recording and staining, we examined the responses of latLFG-PNs to odorants that represent major classes of volatiles released by host plants of M. sexta. All latLFG-PNs were excited when the ipsilateral antenna was stimulated with low concentrations of the monoterpenoid linalool. Dose-response analysis showed that neither other monoterpenoids nor representatives of other classes of host plant volatiles were similarly stimulatory to latLFG-PNs. These findings are consistent with the idea that each glomerulus has a characteristic, limited molecular receptive range.

Original languageEnglish (US)
Pages (from-to)2391-2399
Number of pages9
JournalJournal of Neuroscience
Volume20
Issue number6
StatePublished - Mar 15 2000

Fingerprint

Olfactory Bulb
Neurons
Manduca
Monoterpenes
Automatic Data Processing
Olfactory Nerve
Oviposition
Neuropil
Moths
Odorants
Staining and Labeling

Keywords

  • Antennal lobe
  • Glomeruli
  • Insect
  • Manduca sexta
  • Olfaction
  • Sexual dimorphism of the CNS

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Response characteristics of an identified, sexually dimorphic olfactory glomerulus. / King, Jane Roche; Christensen, Thomas A.; Hildebrand, John G.

In: Journal of Neuroscience, Vol. 20, No. 6, 15.03.2000, p. 2391-2399.

Research output: Contribution to journalArticle

King, Jane Roche ; Christensen, Thomas A. ; Hildebrand, John G. / Response characteristics of an identified, sexually dimorphic olfactory glomerulus. In: Journal of Neuroscience. 2000 ; Vol. 20, No. 6. pp. 2391-2399.
@article{2021479ec3614b639e5537915ff196be,
title = "Response characteristics of an identified, sexually dimorphic olfactory glomerulus",
abstract = "Partitioning of synaptic neuropil into glomeruli is a common feature of primary olfactory centers in most animal species. The functional significance of glomeruli, however, is not yet well understood. The present study is part of our effort to test the hypothesis that each glomerulus is a functional unit dedicated to processing information about a particular odorant or attribute of odor molecules and that the glomerular array constitutes a map of 'odor space.' We investigated the physiological and morphological features of uniglomerular projection neurons (PNs) associated with an identified glomerulus in each antennal lobe of the female sphinx moth, Manduca sexta. This 'lateral large female glomerulus' (latLFG) is sexually dimorphic and therefore may play a female-specific role, such as processing of information about one or more odorants important for orientation of a female to host plants for oviposition. Together with the medial LFG (medLFG), the latLFG resides outside the array of spheroidal ordinary glomeruli, near the entrance of the antennal (olfactory) nerve. Each LFG is innervated by four to five PNs. Using intracellular recording and staining, we examined the responses of latLFG-PNs to odorants that represent major classes of volatiles released by host plants of M. sexta. All latLFG-PNs were excited when the ipsilateral antenna was stimulated with low concentrations of the monoterpenoid linalool. Dose-response analysis showed that neither other monoterpenoids nor representatives of other classes of host plant volatiles were similarly stimulatory to latLFG-PNs. These findings are consistent with the idea that each glomerulus has a characteristic, limited molecular receptive range.",
keywords = "Antennal lobe, Glomeruli, Insect, Manduca sexta, Olfaction, Sexual dimorphism of the CNS",
author = "King, {Jane Roche} and Christensen, {Thomas A.} and Hildebrand, {John G}",
year = "2000",
month = "3",
day = "15",
language = "English (US)",
volume = "20",
pages = "2391--2399",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "6",

}

TY - JOUR

T1 - Response characteristics of an identified, sexually dimorphic olfactory glomerulus

AU - King, Jane Roche

AU - Christensen, Thomas A.

AU - Hildebrand, John G

PY - 2000/3/15

Y1 - 2000/3/15

N2 - Partitioning of synaptic neuropil into glomeruli is a common feature of primary olfactory centers in most animal species. The functional significance of glomeruli, however, is not yet well understood. The present study is part of our effort to test the hypothesis that each glomerulus is a functional unit dedicated to processing information about a particular odorant or attribute of odor molecules and that the glomerular array constitutes a map of 'odor space.' We investigated the physiological and morphological features of uniglomerular projection neurons (PNs) associated with an identified glomerulus in each antennal lobe of the female sphinx moth, Manduca sexta. This 'lateral large female glomerulus' (latLFG) is sexually dimorphic and therefore may play a female-specific role, such as processing of information about one or more odorants important for orientation of a female to host plants for oviposition. Together with the medial LFG (medLFG), the latLFG resides outside the array of spheroidal ordinary glomeruli, near the entrance of the antennal (olfactory) nerve. Each LFG is innervated by four to five PNs. Using intracellular recording and staining, we examined the responses of latLFG-PNs to odorants that represent major classes of volatiles released by host plants of M. sexta. All latLFG-PNs were excited when the ipsilateral antenna was stimulated with low concentrations of the monoterpenoid linalool. Dose-response analysis showed that neither other monoterpenoids nor representatives of other classes of host plant volatiles were similarly stimulatory to latLFG-PNs. These findings are consistent with the idea that each glomerulus has a characteristic, limited molecular receptive range.

AB - Partitioning of synaptic neuropil into glomeruli is a common feature of primary olfactory centers in most animal species. The functional significance of glomeruli, however, is not yet well understood. The present study is part of our effort to test the hypothesis that each glomerulus is a functional unit dedicated to processing information about a particular odorant or attribute of odor molecules and that the glomerular array constitutes a map of 'odor space.' We investigated the physiological and morphological features of uniglomerular projection neurons (PNs) associated with an identified glomerulus in each antennal lobe of the female sphinx moth, Manduca sexta. This 'lateral large female glomerulus' (latLFG) is sexually dimorphic and therefore may play a female-specific role, such as processing of information about one or more odorants important for orientation of a female to host plants for oviposition. Together with the medial LFG (medLFG), the latLFG resides outside the array of spheroidal ordinary glomeruli, near the entrance of the antennal (olfactory) nerve. Each LFG is innervated by four to five PNs. Using intracellular recording and staining, we examined the responses of latLFG-PNs to odorants that represent major classes of volatiles released by host plants of M. sexta. All latLFG-PNs were excited when the ipsilateral antenna was stimulated with low concentrations of the monoterpenoid linalool. Dose-response analysis showed that neither other monoterpenoids nor representatives of other classes of host plant volatiles were similarly stimulatory to latLFG-PNs. These findings are consistent with the idea that each glomerulus has a characteristic, limited molecular receptive range.

KW - Antennal lobe

KW - Glomeruli

KW - Insect

KW - Manduca sexta

KW - Olfaction

KW - Sexual dimorphism of the CNS

UR - http://www.scopus.com/inward/record.url?scp=0034653402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034653402&partnerID=8YFLogxK

M3 - Article

C2 - 10704513

AN - SCOPUS:0034653402

VL - 20

SP - 2391

EP - 2399

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 6

ER -