Response of Plasmodium refractory and susceptible strains of Anopheles gambiae to inoculated Sephadex beads

Susan Paskewitz, Michael A. Riehle

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

A refractory strain of the mosquito, Anopheles gambiae, melanotically encapsulates and destroys malaria parasites in the midgut. Normal development of parasites is observed in a closely related susceptible strain. To examine the basis for the difference in response, the two strains were compared for responses to inoculated Sephadex beads of varying charge. Negatively charged C-25 beads elicited a much stronger reaction in the refractory strain where 49% of the beads were strongly melanized by 24 h, compared with only 5% in the susceptible strain. Male mosquitoes of each strain responded similarly, with 100% of the beads strongly melanized by 24 h in the refractory strain compared with only 5% in the susceptible strain males. A time course revealed that the melanization in refractory but not susceptible mosquitoes increases substantially over time; 91% of C-25 beads were melanized in refractory females at 72 h compared with 9% in the susceptible sample. Neutral G-25 beads and positively charged A-25 beads were melanized in both strains, demonstrating that the capacity to melanize foreign particles is present in susceptible mosquitoes.

Original languageEnglish (US)
Pages (from-to)369-375
Number of pages7
JournalDevelopmental and Comparative Immunology
Volume18
Issue number5
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • Anopheles gambiae
  • Encapsulation
  • Insect immunity
  • Malaria
  • Melanization
  • Plasmodium
  • Sephadex beads

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

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