Responses of bovine lymphocytes to heat shock as modified by breed and antioxidant status.

L. A. Kamwanja, C. C. Chase, J. A. Gutierrez, Vincent Guerriero, T. A. Olson, A. C. Hammond, P. J. Hansen

Research output: Contribution to journalArticle

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Abstract

We tested whether resistance of lymphocytes to heat stress is modified by breed, intracellular glutathione content, and extracellular antioxidants. In the first experiment, lymphocytes from Angus (Bos taurus, non-heat-tolerant), Brahman (B. indicus, heat-tolerant), and Senepol (B. taurus, heat-tolerant) heifers (12 heifers per breed) were cultured at 45 degrees C for 3 h to evaluate thermal killing, at 42 degrees C for 12 h in a 60-h phytohemagglutinin-induced proliferation test, and at 42 degrees C for 1 h to measure induction of heat shock protein 70 (HSP70). Killing at 45 degrees C was affected by breed x temperature (P < .01); the decrease in viability caused by a temperature of 45 degrees C was greater for Angus than for Brahman or Senepol. For phytohemagglutinin-stimulated lymphocytes, heating to 42 degrees C reduced [3H]thymidine incorporation equally for all breeds. Viability at the end of culture was affected (P < .001) by a breed x temperature interaction because the decrease in viability caused by culture at 42 degrees C was greatest for lymphocytes from Angus heifers. Heat shock for 1 h at 42 degrees C caused a two- to threefold increase in intracellular concentrations of HSP70, but there was no interaction of temperature with breed. In another experiment (with lymphocytes harvested from three Holstein cows), buthionine sulfoximine, a glutathione synthesis inhibitor, inhibited (P < .01) proliferation of phytohemagglutinin-stimulated lymphocytes at 38.5 and 42 degrees C. Addition of the antioxidants glutathione or thioredoxin to culture did not reduce the effects of heating to 42 degrees C on proliferation.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalJournal of Animal Science
Volume72
Issue number2
StatePublished - Feb 1994
Externally publishedYes

Fingerprint

heat stress
Shock
lymphocytes
Antioxidants
Hot Temperature
Lymphocytes
breeds
antioxidants
cattle
phytohemagglutinin
heat
Phytohemagglutinins
Senepol
Angus
Glutathione
glutathione
heifers
Temperature
HSP70 Heat-Shock Proteins
Brahman

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Kamwanja, L. A., Chase, C. C., Gutierrez, J. A., Guerriero, V., Olson, T. A., Hammond, A. C., & Hansen, P. J. (1994). Responses of bovine lymphocytes to heat shock as modified by breed and antioxidant status. Journal of Animal Science, 72(2), 438-444.

Responses of bovine lymphocytes to heat shock as modified by breed and antioxidant status. / Kamwanja, L. A.; Chase, C. C.; Gutierrez, J. A.; Guerriero, Vincent; Olson, T. A.; Hammond, A. C.; Hansen, P. J.

In: Journal of Animal Science, Vol. 72, No. 2, 02.1994, p. 438-444.

Research output: Contribution to journalArticle

Kamwanja, LA, Chase, CC, Gutierrez, JA, Guerriero, V, Olson, TA, Hammond, AC & Hansen, PJ 1994, 'Responses of bovine lymphocytes to heat shock as modified by breed and antioxidant status.', Journal of Animal Science, vol. 72, no. 2, pp. 438-444.
Kamwanja LA, Chase CC, Gutierrez JA, Guerriero V, Olson TA, Hammond AC et al. Responses of bovine lymphocytes to heat shock as modified by breed and antioxidant status. Journal of Animal Science. 1994 Feb;72(2):438-444.
Kamwanja, L. A. ; Chase, C. C. ; Gutierrez, J. A. ; Guerriero, Vincent ; Olson, T. A. ; Hammond, A. C. ; Hansen, P. J. / Responses of bovine lymphocytes to heat shock as modified by breed and antioxidant status. In: Journal of Animal Science. 1994 ; Vol. 72, No. 2. pp. 438-444.
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