Resuscitation with lactated Ringer's solution in rats with hemorrhagic shock induces immediate apoptosis

Subrato Deb, Barry Martin, Leon Sun, Paul Ruff, David Burris, Norman Rich, Solenn DeBreux, Brenda Austin, Peter M Rhee

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Background: We hypothesize that different resuscitative fluids may immediately affect the degree of apoptosis after hemorrhagic shock. Methods: Rats (n = 35) were hemorrhaged 27 mL/kg over 5 minutes followed by 1 hour of shock, then resuscitation over 1 hour. The six treatment groups were sham hemorrhage, sham resuscitation, whole blood resuscitation, lactated Ringer's solution (LR) resuscitation with three times the volume bled, sham hemorrhage with LR infusion, and 7.5% hypertonic saline resuscitation (9.7 mL/kg). Liver and small intestine were harvested immediately after resuscitation. Apoptosis was evaluated by using in situ cell death detection method. Results: Resuscitation with LR resulted in a significant increase in small intestinal and liver apoptosis. Animals that received LR infusion without hemorrhage had an increased level of apoptosis in the intestine. Apoptosis in the intestine was observed in both the mucosa and muscularis externa. There was no increase in apoptosis in either organ in the animals resuscitated with sham resuscitation, whole blood, and hypertonic saline compared with the sham hemorrhage group. Conclusion: Resuscitation with LR solution after hemorrhagic shock increased immediate cell death by apoptosis in both the small intestine and liver. There was no significant increase in apoptosis in the animals resuscitated with hypertonic saline, whole blood, or in unresuscitated animals. Thus, the type of resuscitation fluid used may affect the apoptotic cellular response to shock.

Original languageEnglish (US)
Pages (from-to)582-589
Number of pages8
JournalJournal of Trauma
Volume46
Issue number4
StatePublished - Apr 1999
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Resuscitation
Apoptosis
Hemorrhage
Small Intestine
Intestines
Liver
Shock
Cell Death
Ringer's lactate
Ringer's solution
Animal Structures
Mucous Membrane

Keywords

  • Lactated Ringer's solution
  • Liver
  • Programmed cell death
  • Small intestine
  • Tunnel

ASJC Scopus subject areas

  • Surgery

Cite this

Resuscitation with lactated Ringer's solution in rats with hemorrhagic shock induces immediate apoptosis. / Deb, Subrato; Martin, Barry; Sun, Leon; Ruff, Paul; Burris, David; Rich, Norman; DeBreux, Solenn; Austin, Brenda; Rhee, Peter M.

In: Journal of Trauma, Vol. 46, No. 4, 04.1999, p. 582-589.

Research output: Contribution to journalArticle

Deb, S, Martin, B, Sun, L, Ruff, P, Burris, D, Rich, N, DeBreux, S, Austin, B & Rhee, PM 1999, 'Resuscitation with lactated Ringer's solution in rats with hemorrhagic shock induces immediate apoptosis', Journal of Trauma, vol. 46, no. 4, pp. 582-589.
Deb S, Martin B, Sun L, Ruff P, Burris D, Rich N et al. Resuscitation with lactated Ringer's solution in rats with hemorrhagic shock induces immediate apoptosis. Journal of Trauma. 1999 Apr;46(4):582-589.
Deb, Subrato ; Martin, Barry ; Sun, Leon ; Ruff, Paul ; Burris, David ; Rich, Norman ; DeBreux, Solenn ; Austin, Brenda ; Rhee, Peter M. / Resuscitation with lactated Ringer's solution in rats with hemorrhagic shock induces immediate apoptosis. In: Journal of Trauma. 1999 ; Vol. 46, No. 4. pp. 582-589.
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AU - Martin, Barry

AU - Sun, Leon

AU - Ruff, Paul

AU - Burris, David

AU - Rich, Norman

AU - DeBreux, Solenn

AU - Austin, Brenda

AU - Rhee, Peter M

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N2 - Background: We hypothesize that different resuscitative fluids may immediately affect the degree of apoptosis after hemorrhagic shock. Methods: Rats (n = 35) were hemorrhaged 27 mL/kg over 5 minutes followed by 1 hour of shock, then resuscitation over 1 hour. The six treatment groups were sham hemorrhage, sham resuscitation, whole blood resuscitation, lactated Ringer's solution (LR) resuscitation with three times the volume bled, sham hemorrhage with LR infusion, and 7.5% hypertonic saline resuscitation (9.7 mL/kg). Liver and small intestine were harvested immediately after resuscitation. Apoptosis was evaluated by using in situ cell death detection method. Results: Resuscitation with LR resulted in a significant increase in small intestinal and liver apoptosis. Animals that received LR infusion without hemorrhage had an increased level of apoptosis in the intestine. Apoptosis in the intestine was observed in both the mucosa and muscularis externa. There was no increase in apoptosis in either organ in the animals resuscitated with sham resuscitation, whole blood, and hypertonic saline compared with the sham hemorrhage group. Conclusion: Resuscitation with LR solution after hemorrhagic shock increased immediate cell death by apoptosis in both the small intestine and liver. There was no significant increase in apoptosis in the animals resuscitated with hypertonic saline, whole blood, or in unresuscitated animals. Thus, the type of resuscitation fluid used may affect the apoptotic cellular response to shock.

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