Reticulated platelet percentages in term and preterm neonates

Matthew A. Saxonhouse, Martha C. Sola, Karen M. Pastos, Mark E. Ignatz, Alan D. Hutson, Robert D. Christensen, Lisa M Rimsza

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objectives: The authors aimed to determine whether their reticulated platelet percentage (RP%) analysis technique was suitable for use in term and preterm neonates and to characterize RP% values among nonthrombocytopenic neonates. Methods: The authors modified a whole blood method that uses dual-color CD41 staining for platelet gating and thiazole orange for RNA content, combined with RNase treatment of half the sample to subtract non-RNA fluorescence. The RP% was measured in samples from 10 healthy adults and then a longitudinal study was performed in 15 nonthrombocytopenic preterm neonates on days of life 0 to 1, 2 to 5, 6 to 10, and then weekly until day 28. The authors also performed a cross-sectional study of RP% in 22 nonthrombocytopenic neonates of different gestational age (GA) and post-conceptional age (PCA). Results: Overall, neonates had a higher RP% (2.7 ± 1.6%) than adults (1.1 ± 0.5%; P < 0.01). In preterm neonates, an increase in the RP% occurred between days 0 and 1 (3.3 ± 1.3%) and days 2 and 5 (5.1 ± 1.8%; P = 0.003). By days 6 to 10, the RP% decreased to 3.2 ± 1.1% and remained unchanged throughout the rest of the study period. In neonates less than 7 days old, an inverse relationship was observed between RP% and GA (n = 20, r = -0.70; P = 0.0005). A correlation between RP% and PCA was not seen in neonates 7 days of age or older. Conclusions: This method for determining RP% is suitable for use in term and preterm neonates. In preterm infants, the RP% significantly increases over the first 2 to 5 days of life and then decreases to a stable level over the first 28 days. RP% is generally higher in neonates than in adults. Among preterm infants in the first week of life, the RP% is inversely related to GA.

Original languageEnglish (US)
Pages (from-to)797-802
Number of pages6
JournalJournal of Pediatric Hematology/Oncology
Volume26
Issue number12
StatePublished - Dec 2004

Fingerprint

Blood Platelets
Newborn Infant
Gestational Age
Premature Infants
Ribonucleases
Longitudinal Studies
Color
Cross-Sectional Studies
Fluorescence
RNA
Staining and Labeling

Keywords

  • Reticulated platelet percentages
  • Thrombocytopenia
  • Thrombopoiesis

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Oncology
  • Hematology

Cite this

Saxonhouse, M. A., Sola, M. C., Pastos, K. M., Ignatz, M. E., Hutson, A. D., Christensen, R. D., & Rimsza, L. M. (2004). Reticulated platelet percentages in term and preterm neonates. Journal of Pediatric Hematology/Oncology, 26(12), 797-802.

Reticulated platelet percentages in term and preterm neonates. / Saxonhouse, Matthew A.; Sola, Martha C.; Pastos, Karen M.; Ignatz, Mark E.; Hutson, Alan D.; Christensen, Robert D.; Rimsza, Lisa M.

In: Journal of Pediatric Hematology/Oncology, Vol. 26, No. 12, 12.2004, p. 797-802.

Research output: Contribution to journalArticle

Saxonhouse, MA, Sola, MC, Pastos, KM, Ignatz, ME, Hutson, AD, Christensen, RD & Rimsza, LM 2004, 'Reticulated platelet percentages in term and preterm neonates', Journal of Pediatric Hematology/Oncology, vol. 26, no. 12, pp. 797-802.
Saxonhouse MA, Sola MC, Pastos KM, Ignatz ME, Hutson AD, Christensen RD et al. Reticulated platelet percentages in term and preterm neonates. Journal of Pediatric Hematology/Oncology. 2004 Dec;26(12):797-802.
Saxonhouse, Matthew A. ; Sola, Martha C. ; Pastos, Karen M. ; Ignatz, Mark E. ; Hutson, Alan D. ; Christensen, Robert D. ; Rimsza, Lisa M. / Reticulated platelet percentages in term and preterm neonates. In: Journal of Pediatric Hematology/Oncology. 2004 ; Vol. 26, No. 12. pp. 797-802.
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AU - Saxonhouse, Matthew A.

AU - Sola, Martha C.

AU - Pastos, Karen M.

AU - Ignatz, Mark E.

AU - Hutson, Alan D.

AU - Christensen, Robert D.

AU - Rimsza, Lisa M

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N2 - Objectives: The authors aimed to determine whether their reticulated platelet percentage (RP%) analysis technique was suitable for use in term and preterm neonates and to characterize RP% values among nonthrombocytopenic neonates. Methods: The authors modified a whole blood method that uses dual-color CD41 staining for platelet gating and thiazole orange for RNA content, combined with RNase treatment of half the sample to subtract non-RNA fluorescence. The RP% was measured in samples from 10 healthy adults and then a longitudinal study was performed in 15 nonthrombocytopenic preterm neonates on days of life 0 to 1, 2 to 5, 6 to 10, and then weekly until day 28. The authors also performed a cross-sectional study of RP% in 22 nonthrombocytopenic neonates of different gestational age (GA) and post-conceptional age (PCA). Results: Overall, neonates had a higher RP% (2.7 ± 1.6%) than adults (1.1 ± 0.5%; P < 0.01). In preterm neonates, an increase in the RP% occurred between days 0 and 1 (3.3 ± 1.3%) and days 2 and 5 (5.1 ± 1.8%; P = 0.003). By days 6 to 10, the RP% decreased to 3.2 ± 1.1% and remained unchanged throughout the rest of the study period. In neonates less than 7 days old, an inverse relationship was observed between RP% and GA (n = 20, r = -0.70; P = 0.0005). A correlation between RP% and PCA was not seen in neonates 7 days of age or older. Conclusions: This method for determining RP% is suitable for use in term and preterm neonates. In preterm infants, the RP% significantly increases over the first 2 to 5 days of life and then decreases to a stable level over the first 28 days. RP% is generally higher in neonates than in adults. Among preterm infants in the first week of life, the RP% is inversely related to GA.

AB - Objectives: The authors aimed to determine whether their reticulated platelet percentage (RP%) analysis technique was suitable for use in term and preterm neonates and to characterize RP% values among nonthrombocytopenic neonates. Methods: The authors modified a whole blood method that uses dual-color CD41 staining for platelet gating and thiazole orange for RNA content, combined with RNase treatment of half the sample to subtract non-RNA fluorescence. The RP% was measured in samples from 10 healthy adults and then a longitudinal study was performed in 15 nonthrombocytopenic preterm neonates on days of life 0 to 1, 2 to 5, 6 to 10, and then weekly until day 28. The authors also performed a cross-sectional study of RP% in 22 nonthrombocytopenic neonates of different gestational age (GA) and post-conceptional age (PCA). Results: Overall, neonates had a higher RP% (2.7 ± 1.6%) than adults (1.1 ± 0.5%; P < 0.01). In preterm neonates, an increase in the RP% occurred between days 0 and 1 (3.3 ± 1.3%) and days 2 and 5 (5.1 ± 1.8%; P = 0.003). By days 6 to 10, the RP% decreased to 3.2 ± 1.1% and remained unchanged throughout the rest of the study period. In neonates less than 7 days old, an inverse relationship was observed between RP% and GA (n = 20, r = -0.70; P = 0.0005). A correlation between RP% and PCA was not seen in neonates 7 days of age or older. Conclusions: This method for determining RP% is suitable for use in term and preterm neonates. In preterm infants, the RP% significantly increases over the first 2 to 5 days of life and then decreases to a stable level over the first 28 days. RP% is generally higher in neonates than in adults. Among preterm infants in the first week of life, the RP% is inversely related to GA.

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