Retinal dynamics underlie its switch from inverse agonist to agonist during rhodopsin activation

Andrey V. Struts, Gilmar F J Salgado, Karina Martínez-Mayorga, Michael F Brown

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

X-ray and magnetic resonance approaches, though central to studies of G protein-coupled receptor (GPCR)-mediated signaling, cannot address GPCR protein dynamics or plasticity. Here we show that solid-state 2H NMR relaxation elucidates picosecond-to-nanosecond-timescale motions of the retinal ligand that influence larger-scale functional dynamics of rhodopsin in membranes. We propose a multiscale activation mechanism whereby retinal initiates collective helix fluctuations in the meta I-meta II equilibrium on the microsecond-to-millisecond timescale.

Original languageEnglish (US)
Pages (from-to)392-394
Number of pages3
JournalNature Structural and Molecular Biology
Volume18
Issue number3
DOIs
StatePublished - Mar 2011

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Rhodopsin
G-Protein-Coupled Receptors
Magnetic Resonance Spectroscopy
X-Rays
Ligands
Membranes
Proteins

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Retinal dynamics underlie its switch from inverse agonist to agonist during rhodopsin activation. / Struts, Andrey V.; Salgado, Gilmar F J; Martínez-Mayorga, Karina; Brown, Michael F.

In: Nature Structural and Molecular Biology, Vol. 18, No. 3, 03.2011, p. 392-394.

Research output: Contribution to journalArticle

Struts, Andrey V. ; Salgado, Gilmar F J ; Martínez-Mayorga, Karina ; Brown, Michael F. / Retinal dynamics underlie its switch from inverse agonist to agonist during rhodopsin activation. In: Nature Structural and Molecular Biology. 2011 ; Vol. 18, No. 3. pp. 392-394.
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