Retinoic acid-induced changes in epidermal growth factor binding and in biological responses mediated by phorbol ester tumor promoter

Jean M. Lockyer, G. T. Bowden, Michael A. Kelly, Mark R Haussler, Bruce E. Magun

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated the ability of retinoic acid (RA) to alter the binding of epidermal growth factor (EGF) to Rat-1 and Swiss mouse 3T3 cells, the EGF-related induction of ornithine decarboxylase (ODC) activity and DNA synthesis. Pretreatment of 3T3 cells with 10-10-10-5 M RA produced a dose-dependent increase in EGF binding. The increased EGF binding appeared to result from an increased number of EGF receptors and did not occur in Rat-1 cells which, unlike 3T3 cells, possessed measureable amounts of cellular retinoic acid binding protein (CRABP). Both cell lines responded to RA pretreatment with an increase in EGF- and TPA-induced ODC activity, without a concomitant increase in DNA synthesis in either cell line. We suggest that the increase in EGF binding induced by RA in 3T3 cells is apparently unrelated to alterations in EGF-induced biological responses and to the presence of CRABP.

Original languageEnglish (US)
Pages (from-to)171-182
Number of pages12
JournalCancer Letters
Volume22
Issue number2
DOIs
StatePublished - 1984

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Phorbol Esters
Tretinoin
Epidermal Growth Factor
Carcinogens
3T3 Cells
Retinoic Acid Receptors
Ornithine Decarboxylase
Swiss 3T3 Cells
Cell Line
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Retinoic acid-induced changes in epidermal growth factor binding and in biological responses mediated by phorbol ester tumor promoter. / Lockyer, Jean M.; Bowden, G. T.; Kelly, Michael A.; Haussler, Mark R; Magun, Bruce E.

In: Cancer Letters, Vol. 22, No. 2, 1984, p. 171-182.

Research output: Contribution to journalArticle

Lockyer, Jean M. ; Bowden, G. T. ; Kelly, Michael A. ; Haussler, Mark R ; Magun, Bruce E. / Retinoic acid-induced changes in epidermal growth factor binding and in biological responses mediated by phorbol ester tumor promoter. In: Cancer Letters. 1984 ; Vol. 22, No. 2. pp. 171-182.
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