Retroperitoneal inoculation of murine neuroblastoma results in a reliable model for evaluation of the antitumor immune response

Emmanuel Katsanis, Bruce R. Blazar, Maria A. Bausero, Roland Gunther, Peter M. Anderson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To more closely mimic the natural site of human neuroblastoma and the original spontaneous arising paraspinal murine tumor, the authors developed a new model system in which murine neuroblastoma cells (neuro-2a) are implanted directly into the retroperitoneal space. This method of administration resulted in an aggressive and reproducible neuroblastoma model, with death occurring at a median of 20.3 days after tumor implantation using 1 × 106 neuro-2a cells, compared with the intraperitoneal (median, 31 days) and subcutaneous routes (median, 35.1 days) (P < .001). Adoptive transfer of single cell suspensions from livers, spleens, and bone marrows of mice with retroperitoneal tumors into healthy hosts resulted in tumor growth, confirming the presence of metastatic foci in these organs. The retroperitoneal murine neuroblastoma model was used to assess the importance of natural killer (NK) and T cells in regulating the growth of neuro-2a in vivo. T cells played an equally protective role as NK cells; depletion of either T or NK populations significantly decreased survival as compared with undepleted mice. Elimination of both NK and T cells further accelerated mortality of neuro-2a-bearing mice as compared to those depleted of either T or NK populations. The retroperitoneal murine model is a highly relevant in vivo system for preclinical studies of new therapeutic approaches for neuroblastoma.

Original languageEnglish (US)
Pages (from-to)538-542
Number of pages5
JournalJournal of Pediatric Surgery
Volume29
Issue number4
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Neuroblastoma
Natural Killer T-Cells
Neoplasms
Retroperitoneal Space
Adoptive Transfer
Growth
Natural Killer Cells
Population
Suspensions
Spleen
Bone Marrow
T-Lymphocytes
Survival
Mortality
Liver
Therapeutics

Keywords

  • Neuroblastoma

ASJC Scopus subject areas

  • Surgery

Cite this

Retroperitoneal inoculation of murine neuroblastoma results in a reliable model for evaluation of the antitumor immune response. / Katsanis, Emmanuel; Blazar, Bruce R.; Bausero, Maria A.; Gunther, Roland; Anderson, Peter M.

In: Journal of Pediatric Surgery, Vol. 29, No. 4, 1994, p. 538-542.

Research output: Contribution to journalArticle

Katsanis, Emmanuel ; Blazar, Bruce R. ; Bausero, Maria A. ; Gunther, Roland ; Anderson, Peter M. / Retroperitoneal inoculation of murine neuroblastoma results in a reliable model for evaluation of the antitumor immune response. In: Journal of Pediatric Surgery. 1994 ; Vol. 29, No. 4. pp. 538-542.
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