Reverse phase high pressure liquid chromatography for the separation of peptide hormone diastereoisomers

Brent Larsen, V. Viswanatha, Sai Y. Chang, Victor J. Hruby

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Diastereoisomers of specifically labeled oxytocin derivatives were resolved using reverse phase high pressure liquid chromatography. The peptides [1-hemi-DL-[α-2H]cystine]oxytocin, [6-hemi-DL-[α-2H]cystine]oxytocin, [2-DL-[α-2H]tyrosine]oxytocin and [8-DL-[2-13C]leucine]oxytocin were readily separated using the conditions described. The diastereoisomers of the oxytocin analog [3-DL-[2-13C]leucine]oxytocin also demonstrated baseline resolution under the same conditions. The procedure offers the investigator a rapid method for screening synthetic oxytocin peptides for undesirable diastereoisomeric by-products.

Original languageEnglish (US)
Pages (from-to)207-210
Number of pages4
JournalJournal of Chromatographic Science
Volume16
Issue number5
DOIs
StatePublished - May 1978

ASJC Scopus subject areas

  • Analytical Chemistry

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