Reverse transcription polymerase chain reaction (RT-PCR) used for the detection of Taura Syndrome Virus (TSV) in experimentally infected shrimp

L. M. Nunan, B. T. Poulos, Donald V Lightner

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Taura Syndrome Virus (TSV) has adversely affected the shrimp culture industries of the Americas. First recognized in 1992, this vital agent has spread throughout the shrimp growing regions of South and Central America to become established in North America in the short span of 5 yr. Diagnostic methods for TSV include histopathology, bioassay using susceptible Penaeus vannamei as the indicator species and in situ hybridization with TSV specific complimentary DNA (cDNA) gene probes. An additional method for detecting TSV is through the use of reverse transcription polymerase chain reaction (RT-PCR). Two oligonucleotide primers were selected using the sequence information from a cloned cDNA segment of the TSV genome. The primers, designated 9195 and 9992, used in the RT-PCR procedure amplify a 231 base pair (bp) fragment of the cDNA. Using the RT-PCR technique, TSV has been detected in the hemolymph of P. stylirostris and P. vannamei with experimentally induced TSV infections.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalDiseases of Aquatic Organisms
Volume34
Issue number2
StatePublished - Oct 8 1998

Fingerprint

Taura syndrome virus
polymerase chain reaction
virus
shrimp
reverse transcriptase polymerase chain reaction
Litopenaeus vannamei
DNA
shrimp culture
histopathology
DNA primers
detection
Central America
diagnostic techniques
in situ hybridization
indicator species
hemolymph
bioassay
genome
bioassays
probe

Keywords

  • Penaeid shrimp
  • RT-PCR
  • Taura Syndrome Virus (TSV)

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

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abstract = "Taura Syndrome Virus (TSV) has adversely affected the shrimp culture industries of the Americas. First recognized in 1992, this vital agent has spread throughout the shrimp growing regions of South and Central America to become established in North America in the short span of 5 yr. Diagnostic methods for TSV include histopathology, bioassay using susceptible Penaeus vannamei as the indicator species and in situ hybridization with TSV specific complimentary DNA (cDNA) gene probes. An additional method for detecting TSV is through the use of reverse transcription polymerase chain reaction (RT-PCR). Two oligonucleotide primers were selected using the sequence information from a cloned cDNA segment of the TSV genome. The primers, designated 9195 and 9992, used in the RT-PCR procedure amplify a 231 base pair (bp) fragment of the cDNA. Using the RT-PCR technique, TSV has been detected in the hemolymph of P. stylirostris and P. vannamei with experimentally induced TSV infections.",
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