Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles

Jinghua Duan, Heidi M. Mansour, Yangde Zhang, Xingming Deng, Yuxiang Chen, Jiwei Wang, Yifeng Pan, Jinfeng Zhao

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

Co-encapsulated doxorubicin (DOX) and curcumin (CUR) in poly(butyl cyanoacrylate) nanoparticles (PBCA-NPs) were prepared with emulsion polymerization and interfacial polymerization. The mean particle size and mean zeta potential of CUR-DOX-PBCA-NPs were 133 ± 5.34 nm in diameter and +32.23 ± 4.56 mV, respectively. The entrapment efficiencies of doxorubicin and curcumin were 49.98 ± 3.32% and 94.52 ± 3.14%, respectively. Anticancer activities and reversal efficacy of the formulations and various combination approaches were assessed using 3-[4,5-dimethylthiazol-2- yl] 2,5-diphenyltetrazolium bromide assay and western blotting. The results showed that the dual-agent loaded PBCA-NPs system had the similar cytotoxicity to co-administration of two single-agent loaded PBCA-NPs (DOX-PBCA-NPs + CUR-PBCA-NPs), which was slightly higher than that of the free drug combination (DOX + CUR) and one free drug/another agent loaded PBCA-NPs combination (DOX + CUR-PBCA-NPs or CUR + DOX-PBCA-NPs). The simultaneous administration of doxorubicin and curcumin achieved the highest reversal efficacy and down-regulation of P-glycoprotein in MCF-7/ADR cell lines, an MCF-7 breast carcer cell line resistant to adriamycin. Multidrug resistance can be enhanced by combination delivery of encapsulated cytotoxic drugs and reversal agents.

Original languageEnglish (US)
Pages (from-to)193-201
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume426
Issue number1-2
DOIs
StatePublished - Apr 15 2012
Externally publishedYes

Keywords

  • Curcumin
  • Doxorubicin
  • Multidrug resistance
  • Poly(butyl cyanoacrylate) nanoparticles

ASJC Scopus subject areas

  • Pharmaceutical Science

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