Rhodopsin reconstituted into a planar-supported lipid bilayer retains photoactivity after cross-linking polymerization of lipid monomers

Varuni Subramaniam, Isabel D. Alves, Gilmar F J Salgado, Pick Wei Lau, Ronald J. Wysocki, Zdzislaw Salamon, Gordon Tollin, Victor J Hruby, Michael F Brown, Steven S Saavedra

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Transmembrane proteins (TMPs), particularly ion channels and receptors, play key roles in transport and signal transduction. Many of these proteins are pharmacologically important and therefore targets for drug discovery. TMPs can be reconstituted in planar-supported lipid bilayers (PSLBs), which has led to development of TMP-based biosensors and biochips. However, PSLBs composed of natural lipids lack the high stability desired for many technological applications. One strategy is to use synthetic lipid monomers that can be polymerized to form robust bilayers. A key question is how lipid polymerization affects TMP structure and activity. In this study, we have examined the effects of UV polymerization of bis-Sorbylphosphatidylcholine (bis-SorbPC) on the photoactivation of reconstituted bovine rhodopsin (Rho), a model G-protein-coupled receptor. Plasmon-waveguide resonance spectroscopy (PWR) was used to compare the degree of Rho incorporation and activation in fluid and poly(lipid) PSLBs. The results show that reconstitution of Rho into a supported lipid bilayer composed only of bis-SorbPC, followed by photoinduced lipid cross-linking, does not measurably diminish protein function.

Original languageEnglish (US)
Pages (from-to)5320-5321
Number of pages2
JournalJournal of the American Chemical Society
Volume127
Issue number15
DOIs
StatePublished - Apr 20 2005

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Lipid bilayers
Rhodopsin
Lipid Bilayers
Polymerization
Lipids
Monomers
Proteins
Biochips
Signal transduction
Biosensing Techniques
Drug Discovery
G-Protein-Coupled Receptors
Ion Channels
Biosensors
Signal Transduction
Spectrum Analysis
Waveguides
Chemical activation
Spectroscopy
Fluids

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Rhodopsin reconstituted into a planar-supported lipid bilayer retains photoactivity after cross-linking polymerization of lipid monomers. / Subramaniam, Varuni; Alves, Isabel D.; Salgado, Gilmar F J; Lau, Pick Wei; Wysocki, Ronald J.; Salamon, Zdzislaw; Tollin, Gordon; Hruby, Victor J; Brown, Michael F; Saavedra, Steven S.

In: Journal of the American Chemical Society, Vol. 127, No. 15, 20.04.2005, p. 5320-5321.

Research output: Contribution to journalArticle

Subramaniam, Varuni ; Alves, Isabel D. ; Salgado, Gilmar F J ; Lau, Pick Wei ; Wysocki, Ronald J. ; Salamon, Zdzislaw ; Tollin, Gordon ; Hruby, Victor J ; Brown, Michael F ; Saavedra, Steven S. / Rhodopsin reconstituted into a planar-supported lipid bilayer retains photoactivity after cross-linking polymerization of lipid monomers. In: Journal of the American Chemical Society. 2005 ; Vol. 127, No. 15. pp. 5320-5321.
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