Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti: Cloning and expression

D. Q.D. Pham, B. J. Blachuta, H. Nichol, J. J. Winzerling

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are α2β2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.

Original languageEnglish (US)
Pages (from-to)1037-1044
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2002

Keywords

  • Aedes aegypti
  • Mosquito
  • R1
  • R2
  • Ribonucleotide reductase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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