Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti: Cloning and expression

D. Q D Pham, B. J. Blachuta, H. Nichol, Joy Winzerling

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are α2β2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.

Original languageEnglish (US)
Pages (from-to)1037-1044
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume32
Issue number9
DOIs
StatePublished - Sep 2002

Fingerprint

ribonucleotide reductase
Deoxyribonucleotides
Yellow Fever
Ribonucleotide Reductases
Aedes
Cloning
Aedes aegypti
Culicidae
Organism Cloning
Oxidoreductases
Iron
iron
Allosteric Site
Holoenzymes
active sites
Free Radicals
binding sites
Catalytic Domain
Blood
Complementary DNA

Keywords

  • Aedes aegypti
  • Mosquito
  • R1
  • R2
  • Ribonucleotide reductase

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry

Cite this

Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti : Cloning and expression. / Pham, D. Q D; Blachuta, B. J.; Nichol, H.; Winzerling, Joy.

In: Insect Biochemistry and Molecular Biology, Vol. 32, No. 9, 09.2002, p. 1037-1044.

Research output: Contribution to journalArticle

@article{1fe7a446319d4df7a516b85daa06d2f9,
title = "Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti: Cloning and expression",
abstract = "Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are α2β2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.",
keywords = "Aedes aegypti, Mosquito, R1, R2, Ribonucleotide reductase",
author = "Pham, {D. Q D} and Blachuta, {B. J.} and H. Nichol and Joy Winzerling",
year = "2002",
month = "9",
doi = "10.1016/S0965-1748(02)00041-3",
language = "English (US)",
volume = "32",
pages = "1037--1044",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "9",

}

TY - JOUR

T1 - Ribonucleotide reductase subunits from the yellow fever mosquito, Aedes aegypti

T2 - Cloning and expression

AU - Pham, D. Q D

AU - Blachuta, B. J.

AU - Nichol, H.

AU - Winzerling, Joy

PY - 2002/9

Y1 - 2002/9

N2 - Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are α2β2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.

AB - Ribonucleotide reductase catalyses the de novo synthesis of deoxyribonucleotides. Class I reductases use an iron center to generate a tyrosyl free radical that can initiate formation of the deoxyribonucleotide. These reductases are α2β2 holoenzymes, and the subunits are denoted as R1 and R2. R1 contains the allosteric binding site and the active site, whereas R2 contains a binuclear iron center that initiates formation of the tyrosyl radical. We have cloned and sequenced the cDNAs encoding the R1 and R2 subunit in the yellow fever mosquito, Aedes aegypti. The messages for these proteins are increased in response to blood-feeding.

KW - Aedes aegypti

KW - Mosquito

KW - R1

KW - R2

KW - Ribonucleotide reductase

UR - http://www.scopus.com/inward/record.url?scp=0036713334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036713334&partnerID=8YFLogxK

U2 - 10.1016/S0965-1748(02)00041-3

DO - 10.1016/S0965-1748(02)00041-3

M3 - Article

C2 - 12213240

AN - SCOPUS:0036713334

VL - 32

SP - 1037

EP - 1044

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 9

ER -