RNAi-mediated mortality of the whitefly through transgenic expression of double-stranded RNA homologous to acetylcholinesterase and ecdysone receptor in tobacco plants

Hassan Jamil Malik, Amir Raza, Imran Amin, Jodi A. Scheffler, Brian E. Scheffler, Judith K Brown, Shahid Mansoor

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The whitefly Bemisia tabaci (Genn.) is a pest and vector of plant viruses to crop and ornamental plants worldwide. Using RNA interference (RNAi) to down regulate whitefly genes by expressing their homologous double stranded RNAs in plants has great potential for management of whiteflies to reduce plant virus disease spread. Using a Tobacco rattle virus-derived plasmid for in planta transient expression of double stranded RNA (dsRNA) homologous to the acetylcholinesterase (AChE) and ecdysone receptor (EcR) genes of B. tabaci, resulted in significant adult whitefly mortality. Nicotiana tabacum L. plants expressing dsRNA homologous to B. tabaci AChE and EcR were constructed by fusing sequences derived from both genes. Mortality of adult whiteflies exposed to dsRNA by feeding on N. tabacum plants, compared to non-dsRNA expressing plants, recorded at 24-hr intervals post-ingestion for three days, was >90% and 10%, respectively. Analysis of gene expression by real time quantitative PCR indicated that whitefly mortality was attributable to the down-regulation of both target genes by RNAi. Results indicated that knock down of whitefly genes involved in neuronal transmission and transcriptional activation of developmental genes, has potential as a bio-pesticide to reduce whitefly population size and thereby decrease virus spread.

Original languageEnglish (US)
Article number38469
JournalScientific Reports
Volume6
DOIs
StatePublished - Dec 8 2016

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Hemiptera
Double-Stranded RNA
Acetylcholinesterase
RNA Interference
Tobacco
Mortality
Plant Viruses
Genes
Down-Regulation
Plant RNA
Viruses
Gene Knockdown Techniques
Developmental Genes
Plant Diseases
ecdysone receptor
Virus Diseases
Population Density
Pesticides
Transcriptional Activation
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • General

Cite this

RNAi-mediated mortality of the whitefly through transgenic expression of double-stranded RNA homologous to acetylcholinesterase and ecdysone receptor in tobacco plants. / Malik, Hassan Jamil; Raza, Amir; Amin, Imran; Scheffler, Jodi A.; Scheffler, Brian E.; Brown, Judith K; Mansoor, Shahid.

In: Scientific Reports, Vol. 6, 38469, 08.12.2016.

Research output: Contribution to journalArticle

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