Role for DNA methylation in the control of cell type-specific maspin expression

Bernard W Futscher, Marc M. Oshiro, Ryan J. Wozniak, Nicholas Holtan, Christin L. Hanigan, Hong Duan, Frederick E. Domann

Research output: Contribution to journalArticle

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Abstract

The nucleotide 5-methylcytosine is involved in processes crucial in mammalian development, such as X-chromosome inactivation and gene imprinting. In addition, cytosine methylation has long been speculated to be involved in the establishment and maintenance of cell type-specific expression of developmentally regulated genes; however, it has been difficult to identify clear examples of such genes, particularly in humans. Here we provide evidence that cytosine methylation of the maspin gene (SERPINB5) promoter controls, in part, normal cell type-specific SERPINB5 expression. In normal cells expressing SERPINB5, the SERPINB5 promoter is unmethylated and the promoter region has acetylated histones and an accessible chromatin structure. By contrast, normal cells that do not express SERPINB5 have a completely methylated SERPINB5 promoter with hypoacetylated histones, an inaccessible chromatin structure and a transcriptional repression that is relieved by inhibition of DNA methylation. These findings indicate that cytosine methylation is important in the establishment and maintenance of cell type-restricted gene expression.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalNature Genetics
Volume31
Issue number2
DOIs
StatePublished - Jun 2002

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DNA Methylation
Cytosine
Methylation
Histones
Chromatin
Maintenance
5-Methylcytosine
Genes
X Chromosome Inactivation
X-Linked Genes
Genetic Promoter Regions
Nucleotides
SERPIN-B5
Gene Expression

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Futscher, B. W., Oshiro, M. M., Wozniak, R. J., Holtan, N., Hanigan, C. L., Duan, H., & Domann, F. E. (2002). Role for DNA methylation in the control of cell type-specific maspin expression. Nature Genetics, 31(2), 175-179. https://doi.org/10.1038/ng886

Role for DNA methylation in the control of cell type-specific maspin expression. / Futscher, Bernard W; Oshiro, Marc M.; Wozniak, Ryan J.; Holtan, Nicholas; Hanigan, Christin L.; Duan, Hong; Domann, Frederick E.

In: Nature Genetics, Vol. 31, No. 2, 06.2002, p. 175-179.

Research output: Contribution to journalArticle

Futscher, BW, Oshiro, MM, Wozniak, RJ, Holtan, N, Hanigan, CL, Duan, H & Domann, FE 2002, 'Role for DNA methylation in the control of cell type-specific maspin expression', Nature Genetics, vol. 31, no. 2, pp. 175-179. https://doi.org/10.1038/ng886
Futscher, Bernard W ; Oshiro, Marc M. ; Wozniak, Ryan J. ; Holtan, Nicholas ; Hanigan, Christin L. ; Duan, Hong ; Domann, Frederick E. / Role for DNA methylation in the control of cell type-specific maspin expression. In: Nature Genetics. 2002 ; Vol. 31, No. 2. pp. 175-179.
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