Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow

Denise J. Iuliano, Timothy L. Proulx, Steven S. Saavedra, George A. Truskey

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Forces exerted on adherent calls by fluid shear stresses can cause cell detachment. In this study, we examined the effect of cell spreading, actin fiber density, and the conformation of adsorbed adhesion proteins in promoting cell adhesion after a brief exposure to shear stresses between 5-80 dyne/cm2 in a parallel plate flow channel. Cell spreading led to increased adhesion. Cell spreading and adhesion were greater when the surface contained preadsorbed fibronectin. Spreading was accompanied by formation of cytoskeleton which strengthened the adhesive contact between the cell and surface. Similar numbers of cells were detached from hydrophobic and hydrophilic glass containing preadsorbed in even though the cell binding domain was in different conformations.

Original languageEnglish (US)
Title of host publicationBioprocess Engineering Symposium - 1991
PublisherPubl by ASME
Pages23-25
Number of pages3
ISBN (Print)0791808947
StatePublished - Dec 1 1991
Externally publishedYes
EventWinter Annual Meeting of the American Society of Mechanical Engineers - Atlanta, GA, USA
Duration: Dec 1 1991Dec 6 1991

Publication series

NameAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume21

Other

OtherWinter Annual Meeting of the American Society of Mechanical Engineers
CityAtlanta, GA, USA
Period12/1/9112/6/91

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Iuliano, D. J., Proulx, T. L., Saavedra, S. S., & Truskey, G. A. (1991). Role of adhesion proteins and cytoskeleton in the short-term response of fibroblasts and endothelial cells to steady laminar flow. In Bioprocess Engineering Symposium - 1991 (pp. 23-25). (American Society of Mechanical Engineers, Bioengineering Division (Publication) BED; Vol. 21). Publ by ASME.