Role of ATP and Pi in the mechanism of insulin secretion in the mouse insulinoma betaTC3 cell line.

Klearchos K Papas, R. C. Long, I. Constantinidis, A. Sambanis

Research output: Contribution to journalArticle

Abstract

Understanding the biochemical events associated with glucose-stimulated insulin secretion by pancreatic beta cells is of importance in gaining insight into both the pathophysiology of diabetes and the development of tissue-engineered bioartificial pancreatic substitutes. We have investigated the effects of glucose concentration on the bioenergetic status and on the metabolic and secretory functions exhibited by mouse insulinoma betaTC3 cells entrapped in calcium alginate/poly-L-lysine/alginate (APA) beads. Cells entrapped in APA beads constitute a possible implantable bioartificial pancreas for the long-term treatment of insulin-dependent diabetes mellitus. Our results show that, in entrapped betaTC3 cells, the oxygen consumption rate and the intracellular nucleotide triphosphate levels are unaffected by a step change in glucose concentration from 16 mM to 0 mM for 4.5 h and then back to 16 mM. The intracellular Pi level and the ammonia production rate were doubled, while insulin secretion was decreased 10-fold, upon switching from 16 mM to 0 mM glucose. The implications of these findings in the context of pancreatic beta cell biochemistry and the mechanism of the 'Fuel Hypothesis' are discussed.

Original languageEnglish (US)
JournalBiochemical Journal
Volume326
StatePublished - Sep 15 1997
Externally publishedYes

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Insulinoma
Adenosine Triphosphate
Cells
Insulin
Glucose
Cell Line
Insulin-Secreting Cells
Medical problems
Biochemistry
Type 1 Diabetes Mellitus
Ammonia
Oxygen Consumption
Energy Metabolism
Pancreas
Nucleotides
Tissue
Oxygen
alginate-polylysine-alginate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Role of ATP and Pi in the mechanism of insulin secretion in the mouse insulinoma betaTC3 cell line. / Papas, Klearchos K; Long, R. C.; Constantinidis, I.; Sambanis, A.

In: Biochemical Journal, Vol. 326, 15.09.1997.

Research output: Contribution to journalArticle

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