Role of ATP and P(i) in the mechanism of insulin secretion in the mouse insulinoma βTC3 cell line

Klearchos K Papas, Jr Long R.C., I. Constantinidis, A. Sambanis

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Understanding the biochemical events associated with glucose-stimulated insulin secretion by pancreatic β cells is of importance in gaining insight into both the pathophysiology of diabetes and the development of tissue-engineered bioartificial pancreatic substitutes. We have investigated the effects of glucose concentration on the bioenergetic status and on the metabolic and secretory functions exhibited by mouse insulinoma βTC3 cells entrapped in calcium alginate/poly-L-lysine/alginate (APA) beads. Cells entrapped in APA beads constitute a possible implantable bioartificial pancreas for the long-term treatment of insulin-dependent diabetes mellitus. Our results show that, in entrapped βTC3 cells, the oxygen consumption rate and the intracellular nucleotide triphosphate levels are unaffected by a step change in glucose concentration from 16 mM to 0 mM for 4.5 h and then back to 16 mM. The intracellular P(i) level and the ammonia production rate were doubled, while insulin secretion was decreased 10-fold, upon switching from 16 mM to 0 mM glucose. The implications of these findings in the context of pancreatic β cell biochemistry and the mechanism of the 'Fuel Hypothesis' are discussed.

Original languageEnglish (US)
Pages (from-to)807-814
Number of pages8
JournalBiochemical Journal
Volume326
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Insulinoma
Adenosine Triphosphate
Cells
Insulin
Glucose
Cell Line
Medical problems
Biochemistry
Ammonia
Type 1 Diabetes Mellitus
Nucleotides
Oxygen Consumption
Energy Metabolism
Tissue
Oxygen
Pancreas
alginate-polylysine-alginate

ASJC Scopus subject areas

  • Biochemistry

Cite this

Role of ATP and P(i) in the mechanism of insulin secretion in the mouse insulinoma βTC3 cell line. / Papas, Klearchos K; Long R.C., Jr; Constantinidis, I.; Sambanis, A.

In: Biochemical Journal, Vol. 326, No. 3, 1997, p. 807-814.

Research output: Contribution to journalArticle

Papas, KK, Long R.C., J, Constantinidis, I & Sambanis, A 1997, 'Role of ATP and P(i) in the mechanism of insulin secretion in the mouse insulinoma βTC3 cell line', Biochemical Journal, vol. 326, no. 3, pp. 807-814.
Papas, Klearchos K ; Long R.C., Jr ; Constantinidis, I. ; Sambanis, A. / Role of ATP and P(i) in the mechanism of insulin secretion in the mouse insulinoma βTC3 cell line. In: Biochemical Journal. 1997 ; Vol. 326, No. 3. pp. 807-814.
@article{a6676432325643939298a2aff0458f7a,
title = "Role of ATP and P(i) in the mechanism of insulin secretion in the mouse insulinoma βTC3 cell line",
abstract = "Understanding the biochemical events associated with glucose-stimulated insulin secretion by pancreatic β cells is of importance in gaining insight into both the pathophysiology of diabetes and the development of tissue-engineered bioartificial pancreatic substitutes. We have investigated the effects of glucose concentration on the bioenergetic status and on the metabolic and secretory functions exhibited by mouse insulinoma βTC3 cells entrapped in calcium alginate/poly-L-lysine/alginate (APA) beads. Cells entrapped in APA beads constitute a possible implantable bioartificial pancreas for the long-term treatment of insulin-dependent diabetes mellitus. Our results show that, in entrapped βTC3 cells, the oxygen consumption rate and the intracellular nucleotide triphosphate levels are unaffected by a step change in glucose concentration from 16 mM to 0 mM for 4.5 h and then back to 16 mM. The intracellular P(i) level and the ammonia production rate were doubled, while insulin secretion was decreased 10-fold, upon switching from 16 mM to 0 mM glucose. The implications of these findings in the context of pancreatic β cell biochemistry and the mechanism of the 'Fuel Hypothesis' are discussed.",
author = "Papas, {Klearchos K} and {Long R.C.}, Jr and I. Constantinidis and A. Sambanis",
year = "1997",
language = "English (US)",
volume = "326",
pages = "807--814",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "3",

}

TY - JOUR

T1 - Role of ATP and P(i) in the mechanism of insulin secretion in the mouse insulinoma βTC3 cell line

AU - Papas, Klearchos K

AU - Long R.C., Jr

AU - Constantinidis, I.

AU - Sambanis, A.

PY - 1997

Y1 - 1997

N2 - Understanding the biochemical events associated with glucose-stimulated insulin secretion by pancreatic β cells is of importance in gaining insight into both the pathophysiology of diabetes and the development of tissue-engineered bioartificial pancreatic substitutes. We have investigated the effects of glucose concentration on the bioenergetic status and on the metabolic and secretory functions exhibited by mouse insulinoma βTC3 cells entrapped in calcium alginate/poly-L-lysine/alginate (APA) beads. Cells entrapped in APA beads constitute a possible implantable bioartificial pancreas for the long-term treatment of insulin-dependent diabetes mellitus. Our results show that, in entrapped βTC3 cells, the oxygen consumption rate and the intracellular nucleotide triphosphate levels are unaffected by a step change in glucose concentration from 16 mM to 0 mM for 4.5 h and then back to 16 mM. The intracellular P(i) level and the ammonia production rate were doubled, while insulin secretion was decreased 10-fold, upon switching from 16 mM to 0 mM glucose. The implications of these findings in the context of pancreatic β cell biochemistry and the mechanism of the 'Fuel Hypothesis' are discussed.

AB - Understanding the biochemical events associated with glucose-stimulated insulin secretion by pancreatic β cells is of importance in gaining insight into both the pathophysiology of diabetes and the development of tissue-engineered bioartificial pancreatic substitutes. We have investigated the effects of glucose concentration on the bioenergetic status and on the metabolic and secretory functions exhibited by mouse insulinoma βTC3 cells entrapped in calcium alginate/poly-L-lysine/alginate (APA) beads. Cells entrapped in APA beads constitute a possible implantable bioartificial pancreas for the long-term treatment of insulin-dependent diabetes mellitus. Our results show that, in entrapped βTC3 cells, the oxygen consumption rate and the intracellular nucleotide triphosphate levels are unaffected by a step change in glucose concentration from 16 mM to 0 mM for 4.5 h and then back to 16 mM. The intracellular P(i) level and the ammonia production rate were doubled, while insulin secretion was decreased 10-fold, upon switching from 16 mM to 0 mM glucose. The implications of these findings in the context of pancreatic β cell biochemistry and the mechanism of the 'Fuel Hypothesis' are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0030867470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030867470&partnerID=8YFLogxK

M3 - Article

VL - 326

SP - 807

EP - 814

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 3

ER -