Role of catalase in the smooth muscle relaxant actions of sodium azide and cyanamide

Mohammad Shahidullah, Andrew Duncan, Peter D. Stracħan, Komel M. Rafique, Sarah L. Ball, Mark J.W. McPate, Silvia Nelli, William Martin

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The aim of this study was to determine the role of catalase in the smooth muscle relaxant actions of sodium azide and cyanamide. The effects of 3-amino-1,2,4-triazole suggested a role for this enzyme in the relaxant actions of sodium azide on rat aorta and bovine retractor penis muscle and cyanamide on rat aorta. Moreover, results obtained using a difference spectrophotometric assay based upon the oxidation of haemoglobin were consistent with the catalase-dependent oxidation of sodium azide to nitric oxide (NO) and of cyanamide to nitroxyl anion. Surprisingly, however, no free nitric oxide or nitroxyl was detected in solution using a sensitive electrode. This anomaly might be explained if the stable complexes of catalase with nitric oxide or nitroxyl do not release their respective ligand except to sites of high affinity, such as the haemoglobin employed in the difference spectrophotometric assay, or indeed, the soluble guanylate cyclase within the smooth muscle.

Original languageEnglish (US)
Pages (from-to)93-101
Number of pages9
JournalEuropean Journal of Pharmacology
Volume435
Issue number1
DOIs
StatePublished - Jan 18 2002
Externally publishedYes

Keywords

  • Aorta, rat
  • Catalase
  • Nitric oxide (NO)
  • Nitrovasodilator
  • Nitroxyl anion
  • Retractor penis muscle, bovine

ASJC Scopus subject areas

  • Pharmacology

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  • Cite this

    Shahidullah, M., Duncan, A., Stracħan, P. D., Rafique, K. M., Ball, S. L., McPate, M. J. W., Nelli, S., & Martin, W. (2002). Role of catalase in the smooth muscle relaxant actions of sodium azide and cyanamide. European Journal of Pharmacology, 435(1), 93-101. https://doi.org/10.1016/S0014-2999(01)01540-0