Role of nuclear protein kinase C in the mitogenic response to platelet-derived growth factor

Alan P. Fields, Greg Tyler, Andrew Kraft, W. Stratford May

Research output: Contribution to journalArticle

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Abstract

We have assessed the involvement of nuclear envelope protein phosphorylation in the mitogenic response to platelet-derived growth factor (PDGF) in NIH/3T3 fibroblasts. We find that stimulation of quiescent NIH/3T3 cells with PDGF or with the mitogenic protein kinase C (PKC) activators phorbol 12-myristate 13-acetate (PMA) or bryostatin 1 (bryo) leads to rapid, dose-dependent phosphorylation of several nuclear envelope polypeptides. The predominant nuclear envelope targets for mitogen-induced phosphorylation are immunologically identified as the nuclear envelope lamins. All three lamin species (A, B and C) are phosphorylated in response to PMA or bryo, while lamins A and C are preferentially phosphorylated in response to PDGF. Phosphopeptide mapping and phosphoamino acid analysis indicate that similar serine sites on the lamins are phosphorylated in response to PDGF, PMA and bryo. Both mitogenicity and lamina phosphorylation induced by these mitogens can be inhibited by the selective PKC inhibitor staurosporine at 2 nM. Treatment of quiescent NIH/3T3 cells with PDGF, PMA or bryo leads to rapid translocation of PKC to the nuclear envelope. These data indicate that rapid nuclear events, including translocation of cytosolic PKC to the nuclear membrane and lamina phosphorylation, may play a role in the transduction of the mitogenic signals of PDGF from the cytoplasm to the nucleus in NIH/3T3 fibroblasts.

Original languageEnglish (US)
Pages (from-to)107-114
Number of pages8
JournalJournal of Cell Science
Volume96
Issue number1
StatePublished - May 1990
Externally publishedYes

Fingerprint

Platelet-Derived Growth Factor
Nuclear Envelope
Nuclear Proteins
Protein Kinase C
Phosphorylation
Acetates
Lamins
NIH 3T3 Cells
Mitogens
Fibroblasts
Nuclear Lamina
Phosphoamino Acids
Lamin Type A
Phosphopeptides
Staurosporine
Protein C Inhibitor
Protein Kinase Inhibitors
Serine
Signal Transduction
Cytoplasm

Keywords

  • Bryostatin 1
  • NIH/3T3 fibroblasts
  • Nuclear lamin phosphorylation
  • Phorbol myristate acetate

ASJC Scopus subject areas

  • Cell Biology

Cite this

Role of nuclear protein kinase C in the mitogenic response to platelet-derived growth factor. / Fields, Alan P.; Tyler, Greg; Kraft, Andrew; May, W. Stratford.

In: Journal of Cell Science, Vol. 96, No. 1, 05.1990, p. 107-114.

Research output: Contribution to journalArticle

Fields, Alan P. ; Tyler, Greg ; Kraft, Andrew ; May, W. Stratford. / Role of nuclear protein kinase C in the mitogenic response to platelet-derived growth factor. In: Journal of Cell Science. 1990 ; Vol. 96, No. 1. pp. 107-114.
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