Role of organic acids in the manganese-independent biobleaching system of bjerkandera sp. Strain BOS55

María Teresa Moreira, Gumersindo Feijoo, Tünde Mester, Pablo Mayorga, Reyes Sierra-Alvarez, Jim A. Field

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Bjerkandera sp. strain BOS55 is a white rot fungus that can bleach EDTA- extracted eucalyptus oxygen. delignified kraft pulp (OKP) without any requirement for manganese. Under manganese-free conditions, additions of simple physiological organic acids (e.g., glycolate, glyoxylate, oxalate, and others) at 1 to 5 mM stimulated brightness gains and pulp delignification two- to threefold compared to results for control cultures not receiving acids. The role of the organic acids in improving the manganese-independent biobleaching was shown not to be due to pH-buffering effects. Instead, the stimulation was attributed to enhanced production of manganese peroxidase (MnP) and lignin peroxidase (LIP) as well as increased physiological concentrations of veratryl alcohol and oxalate. These factors contributed to greatly improved production of superoxide anion radicals, which may have accounted for the more extensive biobleaching. Optimum biobleaching corresponded most to the production of MnP. These results suggest that MnP from Bjerkandera is purposefully produced in the absence of manganese and can possibly function independently of manganese in OKP delignification. LiP probably also contributed to OKP delignification when it was present.

Original languageEnglish (US)
Pages (from-to)2409-2417
Number of pages9
JournalApplied and environmental microbiology
Volume64
Issue number7
DOIs
StatePublished - Jul 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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