Role of polyamines in the function of nuclear transcription factor NF-κB in breast cancer cells

Sophia Zaletok, Nataliya Alexandrova, Nina Berdynskykh, Natalia Ignatenko, Sergiy Gogol, Oleksiy Orlovsky, Nadiya Tregubova, Eugene Gerner, Vasyl Chekhun

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The aim of the work was the investigation of the influence of polyamines on formation of nuclear transcription factor kappa B (NF-kB) complex with specific DNA-sequences nuclear response elements (NRE), and the study of content of p50 and p65 (NF-kB subunits) in breast tumor cells under polyamines metabolism modulation. Methods: The model experiments in vitro were carried out on cultured MCF-7 cells, in vivo experiments - on experimental Ca755 and Walker-256 tumors using surface plasmon resonance (SPR) and Western-blotting analysis. Results: The data have demonstrated that polyamines falicitated p50 - NRE binding. Under conditions of polyamine depletion, an increase of content of both p50 and p65 subunits of NF-kB in the nucleus has been registered (especially that of p50 subunits). Upon addition of α-DFMO + putrescine to the culture medium of MCF-7 cells, the decrease of the levels of p50 and p65 proteins in the nuclei has been observed. Conclusion: Polyamines influence the activity of NF-kB transcription factor in breast tumor cells.

Original languageEnglish (US)
Pages (from-to)221-225
Number of pages5
JournalEksperimentalnaa onkologia
Volume26
Issue number3
StatePublished - Sep 2004

Fingerprint

Polyamines
NF-kappa B
Transcription Factors
Breast Neoplasms
MCF-7 Cells
Response Elements
Transcription Factor RelA
Walkers
Putrescine
Surface Plasmon Resonance
Culture Media
Cultured Cells
Western Blotting
Neoplasms
Proteins

Keywords

  • Breast cancer
  • Nuclear transcription factor kappa B
  • Polyamines
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Cancer Research

Cite this

Zaletok, S., Alexandrova, N., Berdynskykh, N., Ignatenko, N., Gogol, S., Orlovsky, O., ... Chekhun, V. (2004). Role of polyamines in the function of nuclear transcription factor NF-κB in breast cancer cells. Eksperimentalnaa onkologia, 26(3), 221-225.

Role of polyamines in the function of nuclear transcription factor NF-κB in breast cancer cells. / Zaletok, Sophia; Alexandrova, Nataliya; Berdynskykh, Nina; Ignatenko, Natalia; Gogol, Sergiy; Orlovsky, Oleksiy; Tregubova, Nadiya; Gerner, Eugene; Chekhun, Vasyl.

In: Eksperimentalnaa onkologia, Vol. 26, No. 3, 09.2004, p. 221-225.

Research output: Contribution to journalArticle

Zaletok, S, Alexandrova, N, Berdynskykh, N, Ignatenko, N, Gogol, S, Orlovsky, O, Tregubova, N, Gerner, E & Chekhun, V 2004, 'Role of polyamines in the function of nuclear transcription factor NF-κB in breast cancer cells', Eksperimentalnaa onkologia, vol. 26, no. 3, pp. 221-225.
Zaletok S, Alexandrova N, Berdynskykh N, Ignatenko N, Gogol S, Orlovsky O et al. Role of polyamines in the function of nuclear transcription factor NF-κB in breast cancer cells. Eksperimentalnaa onkologia. 2004 Sep;26(3):221-225.
Zaletok, Sophia ; Alexandrova, Nataliya ; Berdynskykh, Nina ; Ignatenko, Natalia ; Gogol, Sergiy ; Orlovsky, Oleksiy ; Tregubova, Nadiya ; Gerner, Eugene ; Chekhun, Vasyl. / Role of polyamines in the function of nuclear transcription factor NF-κB in breast cancer cells. In: Eksperimentalnaa onkologia. 2004 ; Vol. 26, No. 3. pp. 221-225.
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