Role of self carriers in the immune response and tolerance. V. Reversal of trinitrophenyl-modified self suppression of the B-cell response by blocking of H-2 antigens

J. J. Jandinski, J. Li, P. J. Wettstein, Jeffrey A Frelinger, D. W. Scott

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Trinitrophenylated syngeneic spleen cells (TNP-SC) are potent tolerogens of the anti-TNP plaque-forming cell (PFC) response in vivo and in vitro. This unresponsive state requires T cells for both its induction and maintenance. Because H-2K/D-restricted cytotoxic T cells are also induced by exposure to TNP-SC, the authors determined the role(s) of histocompatibility antigens (K, I, and D) in the suppression of the PFC response by TNP-SC. They treated syngeneic TNP-modified stimulator cells with antiserum directed at K-, I-, or D-region determinants and found that blocking of H-2K or D antigens on TNP-SC transformed these tolerogens into immunogens capable of eliciting an anti-TNP PFC response in the absence of extrinsic immunogens like TNP-polymerized flagellin. In H-2(k) or H-2(a(k/d)) mice, only H-2K(k) needs to be blocked on the stimulator cells, whereas H-2K or D recognition was apparent in B10.A(4R) mice. These observations indicate that suppression of the PFC response by TNP-SC shows the same restriction in recognition as does the cytotoxic T-cell response. Furthermore, the authors' results suggest that TNP-I-A is recognized by the helper cells in this system as the intrinsic antigen. When both TNP-K and TNP-I-A are present and available on the same stimulator cell, suppression (via modified K recognition) is dominant over help.

Original languageEnglish (US)
Pages (from-to)133-143
Number of pages11
JournalJournal of Experimental Medicine
Volume151
Issue number1
StatePublished - 1980
Externally publishedYes

Fingerprint

H-2 Antigens
Immune Tolerance
B-Lymphocytes
Spleen
T-Lymphocytes
Antigens
Histocompatibility Antigens
Helper-Inducer T-Lymphocytes
Immune Sera

ASJC Scopus subject areas

  • Immunology

Cite this

Role of self carriers in the immune response and tolerance. V. Reversal of trinitrophenyl-modified self suppression of the B-cell response by blocking of H-2 antigens. / Jandinski, J. J.; Li, J.; Wettstein, P. J.; Frelinger, Jeffrey A; Scott, D. W.

In: Journal of Experimental Medicine, Vol. 151, No. 1, 1980, p. 133-143.

Research output: Contribution to journalArticle

@article{6d57e75fe2374755a695fecec4b3e705,
title = "Role of self carriers in the immune response and tolerance. V. Reversal of trinitrophenyl-modified self suppression of the B-cell response by blocking of H-2 antigens",
abstract = "Trinitrophenylated syngeneic spleen cells (TNP-SC) are potent tolerogens of the anti-TNP plaque-forming cell (PFC) response in vivo and in vitro. This unresponsive state requires T cells for both its induction and maintenance. Because H-2K/D-restricted cytotoxic T cells are also induced by exposure to TNP-SC, the authors determined the role(s) of histocompatibility antigens (K, I, and D) in the suppression of the PFC response by TNP-SC. They treated syngeneic TNP-modified stimulator cells with antiserum directed at K-, I-, or D-region determinants and found that blocking of H-2K or D antigens on TNP-SC transformed these tolerogens into immunogens capable of eliciting an anti-TNP PFC response in the absence of extrinsic immunogens like TNP-polymerized flagellin. In H-2(k) or H-2(a(k/d)) mice, only H-2K(k) needs to be blocked on the stimulator cells, whereas H-2K or D recognition was apparent in B10.A(4R) mice. These observations indicate that suppression of the PFC response by TNP-SC shows the same restriction in recognition as does the cytotoxic T-cell response. Furthermore, the authors' results suggest that TNP-I-A is recognized by the helper cells in this system as the intrinsic antigen. When both TNP-K and TNP-I-A are present and available on the same stimulator cell, suppression (via modified K recognition) is dominant over help.",
author = "Jandinski, {J. J.} and J. Li and Wettstein, {P. J.} and Frelinger, {Jeffrey A} and Scott, {D. W.}",
year = "1980",
language = "English (US)",
volume = "151",
pages = "133--143",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Role of self carriers in the immune response and tolerance. V. Reversal of trinitrophenyl-modified self suppression of the B-cell response by blocking of H-2 antigens

AU - Jandinski, J. J.

AU - Li, J.

AU - Wettstein, P. J.

AU - Frelinger, Jeffrey A

AU - Scott, D. W.

PY - 1980

Y1 - 1980

N2 - Trinitrophenylated syngeneic spleen cells (TNP-SC) are potent tolerogens of the anti-TNP plaque-forming cell (PFC) response in vivo and in vitro. This unresponsive state requires T cells for both its induction and maintenance. Because H-2K/D-restricted cytotoxic T cells are also induced by exposure to TNP-SC, the authors determined the role(s) of histocompatibility antigens (K, I, and D) in the suppression of the PFC response by TNP-SC. They treated syngeneic TNP-modified stimulator cells with antiserum directed at K-, I-, or D-region determinants and found that blocking of H-2K or D antigens on TNP-SC transformed these tolerogens into immunogens capable of eliciting an anti-TNP PFC response in the absence of extrinsic immunogens like TNP-polymerized flagellin. In H-2(k) or H-2(a(k/d)) mice, only H-2K(k) needs to be blocked on the stimulator cells, whereas H-2K or D recognition was apparent in B10.A(4R) mice. These observations indicate that suppression of the PFC response by TNP-SC shows the same restriction in recognition as does the cytotoxic T-cell response. Furthermore, the authors' results suggest that TNP-I-A is recognized by the helper cells in this system as the intrinsic antigen. When both TNP-K and TNP-I-A are present and available on the same stimulator cell, suppression (via modified K recognition) is dominant over help.

AB - Trinitrophenylated syngeneic spleen cells (TNP-SC) are potent tolerogens of the anti-TNP plaque-forming cell (PFC) response in vivo and in vitro. This unresponsive state requires T cells for both its induction and maintenance. Because H-2K/D-restricted cytotoxic T cells are also induced by exposure to TNP-SC, the authors determined the role(s) of histocompatibility antigens (K, I, and D) in the suppression of the PFC response by TNP-SC. They treated syngeneic TNP-modified stimulator cells with antiserum directed at K-, I-, or D-region determinants and found that blocking of H-2K or D antigens on TNP-SC transformed these tolerogens into immunogens capable of eliciting an anti-TNP PFC response in the absence of extrinsic immunogens like TNP-polymerized flagellin. In H-2(k) or H-2(a(k/d)) mice, only H-2K(k) needs to be blocked on the stimulator cells, whereas H-2K or D recognition was apparent in B10.A(4R) mice. These observations indicate that suppression of the PFC response by TNP-SC shows the same restriction in recognition as does the cytotoxic T-cell response. Furthermore, the authors' results suggest that TNP-I-A is recognized by the helper cells in this system as the intrinsic antigen. When both TNP-K and TNP-I-A are present and available on the same stimulator cell, suppression (via modified K recognition) is dominant over help.

UR - http://www.scopus.com/inward/record.url?scp=0018865803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018865803&partnerID=8YFLogxK

M3 - Article

C2 - 6444234

AN - SCOPUS:0018865803

VL - 151

SP - 133

EP - 143

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 1

ER -