Role of serum in stimulation of polymorphonuclear leukocyte, luminol-dependent chemiluminescence by influenza A

P. A. Shult, E. C. Dick, Keith A Joiner, W. W. Busse

Research output: Contribution to journalArticle

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Abstract

Granulocyte membrane perturbation activates oxidative metabolism with the release of highly reactive species (O2-, H2O2, OH·, and 'O2) and emission of light (chemiluminescence (CL)). Using the CL response as a measure of oxidative metabolism, we assayed the effects of influenza A on the granulocyte respiratory burst. Human polymorphonuclear leukocytes (PMNs) were isolated by Ficoll-Hypaque cushioning and dextran sedimentation. The isolated PMNs were incubated with egg-grown influenza A (H3N2) virus, or a medium control, in the presence of 1 μM luminol and fresh autologous (10%). No light emission occurred during the incubation of PMNs with the medium control. Influenza A (33 to 50% egg-infective-doses (EID50):1 PMN) stimulated PMN light emission with a maximal response (48,386 ± 10,764 cpm/106 PMN) occurring at 37°C. The degree of CL was dependent on the virus dose with a diminished response (6,041 ± 3,200 cpm/106 PMN) occurring at a lower infectivity of 10 EID50:1 PMN. Chemiluminescence responses were similar with infective and with noninfective virus particles (heat inactivated, 56°C x 2 h). Fresh serum was necessary for the influenza virus to cause a CL response. A significant correlation (p < 0.01) existed between the level of light emission and the hemagglutination-inhibiting (HI) antibody titer to influenza A of the autologous serum. Virus in the absence of detectable antibody did not stimulate CL. The virus-associated CL was completely inhibited if autologous serum was heated (56°C x 30 min) or if the PMNs were pretreated with cytochalasin B (5 mcg/ml x 5 min). These findings suggest that influenza A-associated PMN CL requires antibody, complement, and phagocytosis. In addition, PMNs incubated with influenza virus and autologous serum showed a marked inhibition (up to 95%) of the CL response to zymosan particles when compared with non-virus treated cells. The degree of inhibition was directly related to the level of HI antibody in the serum. Our data suggest that influenza virus inhibits chemiluminescence by consuming serum factors necessary to opsonize the zymosan particles rather than by a direct virus-induced defect of the PMN.

Original languageEnglish (US)
Pages (from-to)267-272
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume131
Issue number2
StatePublished - 1985
Externally publishedYes

Fingerprint

Luminol
Luminescence
Human Influenza
Neutrophils
Serum
Orthomyxoviridae
Viruses
Light
Zymosan
Antibodies
Hemagglutination
Granulocytes
Ovum
H3N2 Subtype Influenza A Virus
Diatrizoate
Ficoll
Cytochalasin B
Respiratory Burst
Influenza A virus
Dextrans

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Role of serum in stimulation of polymorphonuclear leukocyte, luminol-dependent chemiluminescence by influenza A. / Shult, P. A.; Dick, E. C.; Joiner, Keith A; Busse, W. W.

In: American Review of Respiratory Disease, Vol. 131, No. 2, 1985, p. 267-272.

Research output: Contribution to journalArticle

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abstract = "Granulocyte membrane perturbation activates oxidative metabolism with the release of highly reactive species (O2-, H2O2, OH·, and 'O2) and emission of light (chemiluminescence (CL)). Using the CL response as a measure of oxidative metabolism, we assayed the effects of influenza A on the granulocyte respiratory burst. Human polymorphonuclear leukocytes (PMNs) were isolated by Ficoll-Hypaque cushioning and dextran sedimentation. The isolated PMNs were incubated with egg-grown influenza A (H3N2) virus, or a medium control, in the presence of 1 μM luminol and fresh autologous (10{\%}). No light emission occurred during the incubation of PMNs with the medium control. Influenza A (33 to 50{\%} egg-infective-doses (EID50):1 PMN) stimulated PMN light emission with a maximal response (48,386 ± 10,764 cpm/106 PMN) occurring at 37°C. The degree of CL was dependent on the virus dose with a diminished response (6,041 ± 3,200 cpm/106 PMN) occurring at a lower infectivity of 10 EID50:1 PMN. Chemiluminescence responses were similar with infective and with noninfective virus particles (heat inactivated, 56°C x 2 h). Fresh serum was necessary for the influenza virus to cause a CL response. A significant correlation (p < 0.01) existed between the level of light emission and the hemagglutination-inhibiting (HI) antibody titer to influenza A of the autologous serum. Virus in the absence of detectable antibody did not stimulate CL. The virus-associated CL was completely inhibited if autologous serum was heated (56°C x 30 min) or if the PMNs were pretreated with cytochalasin B (5 mcg/ml x 5 min). These findings suggest that influenza A-associated PMN CL requires antibody, complement, and phagocytosis. In addition, PMNs incubated with influenza virus and autologous serum showed a marked inhibition (up to 95{\%}) of the CL response to zymosan particles when compared with non-virus treated cells. The degree of inhibition was directly related to the level of HI antibody in the serum. Our data suggest that influenza virus inhibits chemiluminescence by consuming serum factors necessary to opsonize the zymosan particles rather than by a direct virus-induced defect of the PMN.",
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AU - Shult, P. A.

AU - Dick, E. C.

AU - Joiner, Keith A

AU - Busse, W. W.

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N2 - Granulocyte membrane perturbation activates oxidative metabolism with the release of highly reactive species (O2-, H2O2, OH·, and 'O2) and emission of light (chemiluminescence (CL)). Using the CL response as a measure of oxidative metabolism, we assayed the effects of influenza A on the granulocyte respiratory burst. Human polymorphonuclear leukocytes (PMNs) were isolated by Ficoll-Hypaque cushioning and dextran sedimentation. The isolated PMNs were incubated with egg-grown influenza A (H3N2) virus, or a medium control, in the presence of 1 μM luminol and fresh autologous (10%). No light emission occurred during the incubation of PMNs with the medium control. Influenza A (33 to 50% egg-infective-doses (EID50):1 PMN) stimulated PMN light emission with a maximal response (48,386 ± 10,764 cpm/106 PMN) occurring at 37°C. The degree of CL was dependent on the virus dose with a diminished response (6,041 ± 3,200 cpm/106 PMN) occurring at a lower infectivity of 10 EID50:1 PMN. Chemiluminescence responses were similar with infective and with noninfective virus particles (heat inactivated, 56°C x 2 h). Fresh serum was necessary for the influenza virus to cause a CL response. A significant correlation (p < 0.01) existed between the level of light emission and the hemagglutination-inhibiting (HI) antibody titer to influenza A of the autologous serum. Virus in the absence of detectable antibody did not stimulate CL. The virus-associated CL was completely inhibited if autologous serum was heated (56°C x 30 min) or if the PMNs were pretreated with cytochalasin B (5 mcg/ml x 5 min). These findings suggest that influenza A-associated PMN CL requires antibody, complement, and phagocytosis. In addition, PMNs incubated with influenza virus and autologous serum showed a marked inhibition (up to 95%) of the CL response to zymosan particles when compared with non-virus treated cells. The degree of inhibition was directly related to the level of HI antibody in the serum. Our data suggest that influenza virus inhibits chemiluminescence by consuming serum factors necessary to opsonize the zymosan particles rather than by a direct virus-induced defect of the PMN.

AB - Granulocyte membrane perturbation activates oxidative metabolism with the release of highly reactive species (O2-, H2O2, OH·, and 'O2) and emission of light (chemiluminescence (CL)). Using the CL response as a measure of oxidative metabolism, we assayed the effects of influenza A on the granulocyte respiratory burst. Human polymorphonuclear leukocytes (PMNs) were isolated by Ficoll-Hypaque cushioning and dextran sedimentation. The isolated PMNs were incubated with egg-grown influenza A (H3N2) virus, or a medium control, in the presence of 1 μM luminol and fresh autologous (10%). No light emission occurred during the incubation of PMNs with the medium control. Influenza A (33 to 50% egg-infective-doses (EID50):1 PMN) stimulated PMN light emission with a maximal response (48,386 ± 10,764 cpm/106 PMN) occurring at 37°C. The degree of CL was dependent on the virus dose with a diminished response (6,041 ± 3,200 cpm/106 PMN) occurring at a lower infectivity of 10 EID50:1 PMN. Chemiluminescence responses were similar with infective and with noninfective virus particles (heat inactivated, 56°C x 2 h). Fresh serum was necessary for the influenza virus to cause a CL response. A significant correlation (p < 0.01) existed between the level of light emission and the hemagglutination-inhibiting (HI) antibody titer to influenza A of the autologous serum. Virus in the absence of detectable antibody did not stimulate CL. The virus-associated CL was completely inhibited if autologous serum was heated (56°C x 30 min) or if the PMNs were pretreated with cytochalasin B (5 mcg/ml x 5 min). These findings suggest that influenza A-associated PMN CL requires antibody, complement, and phagocytosis. In addition, PMNs incubated with influenza virus and autologous serum showed a marked inhibition (up to 95%) of the CL response to zymosan particles when compared with non-virus treated cells. The degree of inhibition was directly related to the level of HI antibody in the serum. Our data suggest that influenza virus inhibits chemiluminescence by consuming serum factors necessary to opsonize the zymosan particles rather than by a direct virus-induced defect of the PMN.

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