Roles of SLP-76, phosphoinositide 3-kinase, and gelsolin in the platelet shape changes initiated by the collagen receptor GPVI/FcRγ-chain complex

H. Falet, K. L. Barkalow, Vadim I Pivniouk, M. J. Barnes, R. S. Gena, J. H. Hartwig

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

How platelet shape change initiated by a collagen-related peptide (CRP) specific for the GPVI/FcRγ-chain complex (GPVI/FcRγ-chain) is coupled to SLP-76, phosphoinositide (PI) 3-kinase, and gelsolin is reported. As shown by video microscopy, platelets rapidly round and grow dynamic filopodial projections that rotate around the periphery of the cell after they contact a CRP-coated surface. Lamellae subsequently spread between the projections. All the actin-driven shape changes require SLP-76 expression. SLP-76 is essential for the Ca++ mobilization induced by CRP, whereas PI 3-kinase only modulates it. The extension of lamellae requires net actin assembly and an exposure of actin filament barbed ends downstream of PI 3-kinase. Gelsolin expression is also required for the extension of lamellae, but not for the formation of filopodia. Altogether, the data describe the role of SLP-76 in the platelet activation initiated by GPVI/FcRγ-chain and the roles of PI 3-kinase and gelsolin in lamellae spreading. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)3786-3792
Number of pages7
JournalBlood
Volume96
Issue number12
StatePublished - Dec 1 2000
Externally publishedYes

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Gelsolin
1-Phosphatidylinositol 4-Kinase
Platelets
Phosphatidylinositols
Phosphotransferases
Blood Platelets
Actins
Video Microscopy
Pseudopodia
Platelet Activation
Actin Cytoskeleton
Microscopic examination
Chemical activation
platelet membrane glycoprotein VI
collagen-related peptide

ASJC Scopus subject areas

  • Hematology

Cite this

Roles of SLP-76, phosphoinositide 3-kinase, and gelsolin in the platelet shape changes initiated by the collagen receptor GPVI/FcRγ-chain complex. / Falet, H.; Barkalow, K. L.; Pivniouk, Vadim I; Barnes, M. J.; Gena, R. S.; Hartwig, J. H.

In: Blood, Vol. 96, No. 12, 01.12.2000, p. 3786-3792.

Research output: Contribution to journalArticle

Falet, H. ; Barkalow, K. L. ; Pivniouk, Vadim I ; Barnes, M. J. ; Gena, R. S. ; Hartwig, J. H. / Roles of SLP-76, phosphoinositide 3-kinase, and gelsolin in the platelet shape changes initiated by the collagen receptor GPVI/FcRγ-chain complex. In: Blood. 2000 ; Vol. 96, No. 12. pp. 3786-3792.
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AU - Pivniouk, Vadim I

AU - Barnes, M. J.

AU - Gena, R. S.

AU - Hartwig, J. H.

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