Roles of two homotetrameric kinesins in sea urchin embryonic cell division

Kitty K. Chui, Gregory C. Rogers, Anna M. Kashina, Karen P. Wedaman, David J. Sharp, Duy T. Nguyen, Fred Wilt, Jonathan M. Scholey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To improve our understanding of the roles of microtubule cross-linking motors in mitosis, we analyzed two sea urchin embryonic kinesin-related proteins. It is striking to note that both of these proteins behave as homotetramers, but one behaves as a more compact molecule than the other. These observations suggest that these two presumptive motors could cross-link microtubules into bundles with different spacing. Both motors localize to mitotic spindles, and antibody microinjection experiments suggest that they have mitotic functions. Thus, one of these kinesin-related proteins may cross-link spindle microtubules into loose bundles that are 'tightened' by the other.

Original languageEnglish (US)
Pages (from-to)38005-38011
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number48
DOIs
StatePublished - Dec 1 2000
Externally publishedYes

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Kinesin
Sea Urchins
Microtubules
Cell Division
Cells
Spindle Apparatus
Proteins
Microinjections
Mitosis
Molecules
Antibodies
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chui, K. K., Rogers, G. C., Kashina, A. M., Wedaman, K. P., Sharp, D. J., Nguyen, D. T., ... Scholey, J. M. (2000). Roles of two homotetrameric kinesins in sea urchin embryonic cell division. Journal of Biological Chemistry, 275(48), 38005-38011. https://doi.org/10.1074/jbc.M005948200

Roles of two homotetrameric kinesins in sea urchin embryonic cell division. / Chui, Kitty K.; Rogers, Gregory C.; Kashina, Anna M.; Wedaman, Karen P.; Sharp, David J.; Nguyen, Duy T.; Wilt, Fred; Scholey, Jonathan M.

In: Journal of Biological Chemistry, Vol. 275, No. 48, 01.12.2000, p. 38005-38011.

Research output: Contribution to journalArticle

Chui, KK, Rogers, GC, Kashina, AM, Wedaman, KP, Sharp, DJ, Nguyen, DT, Wilt, F & Scholey, JM 2000, 'Roles of two homotetrameric kinesins in sea urchin embryonic cell division', Journal of Biological Chemistry, vol. 275, no. 48, pp. 38005-38011. https://doi.org/10.1074/jbc.M005948200
Chui, Kitty K. ; Rogers, Gregory C. ; Kashina, Anna M. ; Wedaman, Karen P. ; Sharp, David J. ; Nguyen, Duy T. ; Wilt, Fred ; Scholey, Jonathan M. / Roles of two homotetrameric kinesins in sea urchin embryonic cell division. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 48. pp. 38005-38011.
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