Root organogenesis from single cells released from the root cap of Medicago sp.

Martha C Hawes, Laura Y. Smith, Martha Stephenson

8 Scopus citations


Root border cells were isolated from alfalfa seedlings, and incubated in culture medium with growth regulators. Alfalfa seedlings yielded 1500±100 cells per root, and initial viability of the cells was 95±5%. Multiple cell divisions occurred in the border cells within two weeks. Cell clusters transferred to solidified medium containing growth regulators developed into rapidly growing, friable callus. When transferred to growth regulator-free medium, some of the calluses generated normal roots.

Original languageEnglish (US)
Pages (from-to)303-308
Number of pages6
JournalPlant Cell, Tissue and Organ Culture
Issue number3
Publication statusPublished - Dec 1991


  • Alfalfa
  • alfalfa tissue culture
  • root border cells
  • root cap
  • sloughed root cap cells

ASJC Scopus subject areas

  • Biotechnology
  • Plant Science

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