Rotavirus stability and inactivation

M. Kolb Estes; D. Y. Graham; E. M. Smith; C. P. Gerba

Rotavirus stability and inactivation

M. Kolb Estes, D. Y. Graham, E. M. Smith, C. P. Gerba

Research output: Contribution to journal › Article › peer-review

Abstract

The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl₂, but was stabilized by heating in 2 M-MgSO₄; reovirus I infectivity was enhanced by heating in MgCl₂. Both SAII and reovirus I were inactivated by freezing in MgCl₂. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.

Original language	English (US)
Pages (from-to)	403-409
Number of pages	7
Journal	Journal of General Virology
Volume	43
Issue number	2
State	Published - Dec 1 1979
Externally published	Yes

ASJC Scopus subject areas

Virology

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title = "Rotavirus stability and inactivation",

abstract = "The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl2, but was stabilized by heating in 2 M-MgSO4; reovirus I infectivity was enhanced by heating in MgCl2. Both SAII and reovirus I were inactivated by freezing in MgCl2. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.",

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AU - Kolb Estes, M.

AU - Graham, D. Y.

AU - Smith, E. M.

AU - Gerba, C. P.

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N2 - The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl2, but was stabilized by heating in 2 M-MgSO4; reovirus I infectivity was enhanced by heating in MgCl2. Both SAII and reovirus I were inactivated by freezing in MgCl2. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.

AB - The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl2, but was stabilized by heating in 2 M-MgSO4; reovirus I infectivity was enhanced by heating in MgCl2. Both SAII and reovirus I were inactivated by freezing in MgCl2. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.

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