Abstract
The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl2, but was stabilized by heating in 2 M-MgSO4; reovirus I infectivity was enhanced by heating in MgCl2. Both SAII and reovirus I were inactivated by freezing in MgCl2. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 403-409 |
| Number of pages | 7 |
| Journal | Journal of General Virology |
| Volume | 43 |
| Issue number | 2 |
| State | Published - 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Virology
Cite this
Rotavirus stability and inactivation. / Kolb Estes, M.; Graham, D. Y.; Smith, E. M.; Gerba, Charles P.
In: Journal of General Virology, Vol. 43, No. 2, 1979, p. 403-409.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rotavirus stability and inactivation
AU - Kolb Estes, M.
AU - Graham, D. Y.
AU - Smith, E. M.
AU - Gerba, Charles P
PY - 1979
Y1 - 1979
N2 - The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl2, but was stabilized by heating in 2 M-MgSO4; reovirus I infectivity was enhanced by heating in MgCl2. Both SAII and reovirus I were inactivated by freezing in MgCl2. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.
AB - The stability of the infectivity of Simian rotavirus, SAII, has been analysed and compared to the stability of reovirus type I. SAII infectivity was stable to freeze-thawing, sonication, incubation at 25°C overnight or at 37°C for 1 h and to treatment with acid, ether, chloroform and Genetron. In contrast to reovirus, the infectivity of SAII was more rapidly inactivated by heating at 50°C. SAII infectivity was inactivated above pH 10.0 and by heating at 50°C in 2 M-MgCl2, but was stabilized by heating in 2 M-MgSO4; reovirus I infectivity was enhanced by heating in MgCl2. Both SAII and reovirus I were inactivated by freezing in MgCl2. These results show that rotaviruses and reoviruses can be distinguished by their patterns of inactivation by physical and chemical agents.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018743696&partnerID=8YFLogxK
M3 - Article
C2 - 39115
AN - SCOPUS:0018743696
VL - 43
SP - 403
EP - 409
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 2
ER -