S-nitrosoglutathione reductase: An important regulator in human asthma

Loretta G. Que, Zhonghui Yang, Jonathan S. Stamler, Njira L. Lugogo, Monica Kraft

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

Rationale: Nitric oxide bioactivity, mediated through the formation of S-nitrosothiols (SNOs), has a significant effect on bronchomotor tone. S-Nitrosoglutathione is an endogenous bronchodilator that is decreased in children with asthmatic respiratory failure and in adults with asthma undergoing segmental airway challenge. Recently we showed that S-nitrosoglutathione reductase (GSNOR) regulates endogenous SNOs. Mice with genetic deletion of GSNOR are protected from airway hyperresponsivity in an allergic asthma model. Objectives: We hypothesized that GSNOR is increased in human asthma and correlates with lung SNO content and airway reactivity. Methods: We recruited 36 subjects with mild asthma with FEV1 88.5 ± 2.3% predicted and 34 healthy control subjects with FEV1 100.7 ± 2.5% predicted. Bronchoalveolar lavage (BAL) was performed in all subjects. Cell counts, differentials, GSNOR activity, and SNO levels were determined in BAL. Measurements and Main Results: SNO content was decreased in asthmatic BAL comparedwith control BAL and correlated inversely with GSNOR expression in BAL cell lysates. Furthermore, GSNOR activity measured from BAL samples was significantly increased in subjects with asthma compared with control subjects and correlated inversely with the provocative concentration of methacholine causing a 20% decrease in FEV1. Conclusions: These findings suggest that GSNOR is an important regulator of airway SNO content and airways hyperresponsiveness in human asthma.

Original languageEnglish (US)
Pages (from-to)226-231
Number of pages6
JournalAmerican journal of respiratory and critical care medicine
Volume180
Issue number3
DOIs
StatePublished - Aug 1 2009

Keywords

  • Airway hyperresponsiveness
  • Asthma
  • S-nitrosoglutathione reductase
  • S-nitrosothiols

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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