Safety and efficacy of megakaryocytes induced from hematopoietic stem cells in murine and nonhuman primate models

Xin Guan, Meng Qin, Yu Zhang, Yanan Wang, Bin Shen, Zhihua Ren, Xinxin Ding, Wei Dai, Yongping Jiang

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Because of a lack of platelet supply and a U.S. Food and Drug Administration-approved platelet growth factor, megakaryocytes have emerged as an effective substitute for alleviating thrombocytopenia. Here, we report the development of an efficient two-stage culture system that is free of stroma, animal components, and genetic manipulations for the production of functional megakaryocytes from hematopoietic stem cells. Safety and functional studies were performed in murine and nonhuman primate models. One human cryopreserved cord blood CD34+ cell could be induced ex vivo to produce up to 1.0 × 104 megakaryocytes that included CD41a+ and CD42b+ cells at 82.4% 6 6.1% and 73.3% 6 8.5% (mean 6 SD), respectively, yielding approximately 650-fold higher cell numbers than reported previously. Induced human megakaryocytic cells were capable of engrafting and producing functional platelets in the murine xenotransplantation model. In the nonhuman primate model, transplantation of primate megakaryocytic progenitors increased platelet count nadir and enhanced hemostatic function with no adverse effects. In addition, primate platelets were released in vivo as early as 3 hours after transplantation with autologous or allogeneic mature megakaryocytes and lasted for more than 48 hours. These results strongly suggest that large-scale induction of functional megakaryocytic cells is applicable for treating thrombocytopenic blood diseases in the clinic.

Original languageEnglish (US)
Pages (from-to)897-909
Number of pages13
JournalStem Cells Translational Medicine
Volume6
Issue number3
DOIs
Publication statusPublished - Mar 2017
Externally publishedYes

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Keywords

  • Expansion and differentiation
  • Hematopoietic stem cells
  • Megakaryocytes
  • Nonhuman primates
  • Thrombocytopenia
  • Transplantation

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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