Safety of microbubbles and transcranial ultrasound in rabbits

William C. Culp, Aliza T. Brown, Leah Hennings, John Lowery, Benjamin C. Culp, Eren Erdem, Paula Roberson, Terry O. Matsunaga

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

The object of this study was to evaluate the safety of large doses of microbubbles and ultrasound administered to the head of rabbits as if they were receiving acute stroke therapy of a similar nature. Materials and Methods: Female New Zealand White rabbits were used, N=24, in three groups 1] n=4 control (no treatment), 2] n=10 bubble control (ultrasound plus aspirin), and 3] n=10 target group (ultrasound plus aspirin plus MRX-815 microbubbles). Group 3 was infused with IV bubbles over 1 hour at 0.16cc/kg. Ultrasound was delivered to the dehaired side of the head during bubble infusion and for 1 additional hour at 0.8 W/cm2 20% pulsed wave. Rabbits survived for 22 to 24 hours, were imaged with computerized tomography and 3 Tesla magnetic resonance imaging including contrast studies, and sacrificed. Tetrazolium (TTC) and Hematoxylin and Eosin (H&E) sections were made for pathological examination. Results: All 24 animals showed absence of bleeding, endothelial damage, EKG abnormalities, stroke, blood-brain-barrier breakdown, or other acute abnormalities. CT and MRI showed no bleeding or signs of stroke, but two animals had mild hydrocephalus. The EKGs showed normal variation in QTc. Rabbit behavior was normal in all. Minimal chronic inflammation unrelated to the study was seen in 5. Two animals were excluded because of protocol violations and replaced during the study. Conclusion: The administered dose of microbubbles and ultrasound demonstrated no detrimental effects on the healthy rabbit animal model.

Original languageEnglish (US)
Title of host publication6th International Symposium on Therapeutic Ultrasound
Pages542-546
Number of pages5
DOIs
StatePublished - Sep 24 2007
Externally publishedYes
Event6TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND - Oxford, United Kingdom
Duration: Aug 30 2007Sep 2 2007

Publication series

NameAIP Conference Proceedings
Volume911
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other6TH INTERNATIONAL SYMPOSIUM ON THERAPEUTIC ULTRASOUND
CountryUnited Kingdom
CityOxford
Period8/30/079/2/07

Keywords

  • Stroke
  • Thrombolysis
  • Ultrasound

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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  • Cite this

    Culp, W. C., Brown, A. T., Hennings, L., Lowery, J., Culp, B. C., Erdem, E., Roberson, P., & Matsunaga, T. O. (2007). Safety of microbubbles and transcranial ultrasound in rabbits. In 6th International Symposium on Therapeutic Ultrasound (pp. 542-546). (AIP Conference Proceedings; Vol. 911). https://doi.org/10.1063/1.2744327