Abstract
The role of salivary epidermal growth factor (EGF) in the maintenance of ileal mucosal integrity was studied by evaluating the effects of sialoadenectomy on luminal EGF levels, ileal tissue resistance (R(t)), and unidirectional flux of [51Cr]EDTA. Mice in groups 1 (SLX) and 2 (SLX + EGF) were subjected to sialoadenectomy, while mice in groups 3 (Sham) and 4 (Sham + EGF) underwent a sham procedure. All animals received normal diet and water, except that EGF (100 ng/ml) was added to water for SLX + EGF and Sham + EGF mice. At seven days after surgery, luminal EGF levels in gastrointestinal segments and ileal R(t) were significantly reduced by sialoadenectomy, which was prevented by EGF supplementation. Unidirectional flux of [51Cr]EDTA was 6- to 22-fold greater in the ileum of sialoadenectomized mice, which was prevented by EGF administration. Results suggest that salivary EGF may be the major source of intestinal EGF, and it may play a role in maintenance of ileal mucosal integrity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2175-2181 |
| Number of pages | 7 |
| Journal | Digestive Diseases and Sciences |
| Volume | 42 |
| Issue number | 10 |
| State | Published - 1997 |
| Externally published | Yes |
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Keywords
- Mucosal protection
- Paracellular permeability
- Saliva
- Tight junctions
ASJC Scopus subject areas
- Gastroenterology
Cite this
Salivary epidermal growth factor plays a role in protection of ileal mucosal integrity. / Rao, R. K.; Thomas, D. W.; Pepperl, S.; Porreca, Frank.
In: Digestive Diseases and Sciences, Vol. 42, No. 10, 1997, p. 2175-2181.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Salivary epidermal growth factor plays a role in protection of ileal mucosal integrity
AU - Rao, R. K.
AU - Thomas, D. W.
AU - Pepperl, S.
AU - Porreca, Frank
PY - 1997
Y1 - 1997
N2 - The role of salivary epidermal growth factor (EGF) in the maintenance of ileal mucosal integrity was studied by evaluating the effects of sialoadenectomy on luminal EGF levels, ileal tissue resistance (R(t)), and unidirectional flux of [51Cr]EDTA. Mice in groups 1 (SLX) and 2 (SLX + EGF) were subjected to sialoadenectomy, while mice in groups 3 (Sham) and 4 (Sham + EGF) underwent a sham procedure. All animals received normal diet and water, except that EGF (100 ng/ml) was added to water for SLX + EGF and Sham + EGF mice. At seven days after surgery, luminal EGF levels in gastrointestinal segments and ileal R(t) were significantly reduced by sialoadenectomy, which was prevented by EGF supplementation. Unidirectional flux of [51Cr]EDTA was 6- to 22-fold greater in the ileum of sialoadenectomized mice, which was prevented by EGF administration. Results suggest that salivary EGF may be the major source of intestinal EGF, and it may play a role in maintenance of ileal mucosal integrity.
AB - The role of salivary epidermal growth factor (EGF) in the maintenance of ileal mucosal integrity was studied by evaluating the effects of sialoadenectomy on luminal EGF levels, ileal tissue resistance (R(t)), and unidirectional flux of [51Cr]EDTA. Mice in groups 1 (SLX) and 2 (SLX + EGF) were subjected to sialoadenectomy, while mice in groups 3 (Sham) and 4 (Sham + EGF) underwent a sham procedure. All animals received normal diet and water, except that EGF (100 ng/ml) was added to water for SLX + EGF and Sham + EGF mice. At seven days after surgery, luminal EGF levels in gastrointestinal segments and ileal R(t) were significantly reduced by sialoadenectomy, which was prevented by EGF supplementation. Unidirectional flux of [51Cr]EDTA was 6- to 22-fold greater in the ileum of sialoadenectomized mice, which was prevented by EGF administration. Results suggest that salivary EGF may be the major source of intestinal EGF, and it may play a role in maintenance of ileal mucosal integrity.
KW - Mucosal protection
KW - Paracellular permeability
KW - Saliva
KW - Tight junctions
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UR - http://www.scopus.com/inward/citedby.url?scp=0030731514&partnerID=8YFLogxK
M3 - Article
C2 - 9365155
AN - SCOPUS:0030731514
VL - 42
SP - 2175
EP - 2181
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 10
ER -