Salt cress. A halophyte and cryophyte arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles

Günsu Inan, Quan Zhang, Pinghua Li, Zenglan Wang, Ziyi Cao, Hui Zhang, Changqing Zhang, Tanya M. Quist, S. Mark Goodwin, Jianhua Zhu, Huazhong Shi, Barbara Damsz, Tarif Charbaji, Qingqiu Gong, Shisong Ma, Mark Fredricksen, David W. Galbraith, Matthew A. Jenks, David Rhodes, Paul M. HasegawaHans J. Bohnert, Robert J. Joly, Ray A. Bressan, Jian Kang Zhu

Research output: Contribution to journalArticle

310 Citations (Scopus)

Abstract

Salt cress (Thellungiella halophila) is a small winter annual crucifer with a short life cycle. It has a small genome (about 2 x Arabidopsis) with high sequence identity (average 92%) with Arabidopsis, and can be genetically transformed by the simple floral dip procedure. It is capable of copious seed production. Salt cress is an extremophile native to harsh environments and can reproduce after exposure to extreme salinity (500 mM NaCl) or cold to -15°C. It is a typical halophyte that accumulates NaCl at controlled rates and also dramatic levels of Pro (>150 mM) during exposure to high salinity. Stomata of salt cress are distributed on the leaf surface at higher density, but are less open than the stomata of Arabidopsis and respond to salt stress by closing more tightly. Leaves of salt cress are more succulent-like, have a second layer of palisade mesophyll cells, and are frequently shed during extreme salt stress. Roots of salt cress develop both an extra endodermis and cortex cell layer compared to Arabidopsis. Salt cress, although salt and cold tolerant, is not exceptionally tolerant of soil desiccation. We have isolated several ethyl methanesulfonate mutants of salt cress that have reduced salinity tolerance, which provide evidence that salt tolerance in this halophyte can be significantly affected by individual genetic loci. Analysis of salt cress expressed sequence tags provides evidence for the presence of paralogs, missing in the Arabidopsis genome, and for genes with abiotic stress-relevant functions. Hybridizations of salt cress RNA targets to an Arabidopsis whole-genome oligonucleotide array indicate that commonly stress-associated transcripts are expressed at a noticeably higher level in unstressed salt cress plants and are induced rapidly under stress. Efficient transformation of salt cress allows for simple gene exchange between Arabidopsis and salt cress. In addition, the generation of T-DNA-tagged mutant collections of salt cress, already in progress, will open the door to a new era of forward and reverse genetic studies of extremophile plant biology.

Original languageEnglish (US)
Pages (from-to)1718-1737
Number of pages20
JournalPlant Physiology
Volume135
Issue number3
DOIs
StatePublished - Jul 2004

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Brassicaceae
Growth and Development
Arabidopsis
molecular genetics
Molecular Biology
growth and development
Salts
salts
Salinity
salinity
Extremophiles
extremophiles
stomata
Genome
salt stress
genome
seed productivity
ethyl methanesulfonate
Halophila
Mesophyll Cells

ASJC Scopus subject areas

  • Plant Science

Cite this

Salt cress. A halophyte and cryophyte arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. / Inan, Günsu; Zhang, Quan; Li, Pinghua; Wang, Zenglan; Cao, Ziyi; Zhang, Hui; Zhang, Changqing; Quist, Tanya M.; Goodwin, S. Mark; Zhu, Jianhua; Shi, Huazhong; Damsz, Barbara; Charbaji, Tarif; Gong, Qingqiu; Ma, Shisong; Fredricksen, Mark; Galbraith, David W.; Jenks, Matthew A.; Rhodes, David; Hasegawa, Paul M.; Bohnert, Hans J.; Joly, Robert J.; Bressan, Ray A.; Zhu, Jian Kang.

In: Plant Physiology, Vol. 135, No. 3, 07.2004, p. 1718-1737.

Research output: Contribution to journalArticle

Inan, G, Zhang, Q, Li, P, Wang, Z, Cao, Z, Zhang, H, Zhang, C, Quist, TM, Goodwin, SM, Zhu, J, Shi, H, Damsz, B, Charbaji, T, Gong, Q, Ma, S, Fredricksen, M, Galbraith, DW, Jenks, MA, Rhodes, D, Hasegawa, PM, Bohnert, HJ, Joly, RJ, Bressan, RA & Zhu, JK 2004, 'Salt cress. A halophyte and cryophyte arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles', Plant Physiology, vol. 135, no. 3, pp. 1718-1737. https://doi.org/10.1104/pp.104.041723
Inan, Günsu ; Zhang, Quan ; Li, Pinghua ; Wang, Zenglan ; Cao, Ziyi ; Zhang, Hui ; Zhang, Changqing ; Quist, Tanya M. ; Goodwin, S. Mark ; Zhu, Jianhua ; Shi, Huazhong ; Damsz, Barbara ; Charbaji, Tarif ; Gong, Qingqiu ; Ma, Shisong ; Fredricksen, Mark ; Galbraith, David W. ; Jenks, Matthew A. ; Rhodes, David ; Hasegawa, Paul M. ; Bohnert, Hans J. ; Joly, Robert J. ; Bressan, Ray A. ; Zhu, Jian Kang. / Salt cress. A halophyte and cryophyte arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles. In: Plant Physiology. 2004 ; Vol. 135, No. 3. pp. 1718-1737.
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AU - Inan, Günsu

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AU - Li, Pinghua

AU - Wang, Zenglan

AU - Cao, Ziyi

AU - Zhang, Hui

AU - Zhang, Changqing

AU - Quist, Tanya M.

AU - Goodwin, S. Mark

AU - Zhu, Jianhua

AU - Shi, Huazhong

AU - Damsz, Barbara

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AU - Gong, Qingqiu

AU - Ma, Shisong

AU - Fredricksen, Mark

AU - Galbraith, David W.

AU - Jenks, Matthew A.

AU - Rhodes, David

AU - Hasegawa, Paul M.

AU - Bohnert, Hans J.

AU - Joly, Robert J.

AU - Bressan, Ray A.

AU - Zhu, Jian Kang

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