Scanning tunneling microscopy of cytoskeletal proteins: Microtubules and intermediate filaments

Stuart Hameroff, Yovana Simic-Krstic, Lawrence Vernetti, Y. C. Lee, Dror Sarid, Jerome Wiedmann, Virgil Elings, Kevin Kjoller, Robert McCuskey

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Direct STM observation of native biomolecules has proven feasible. In this study we have used STM to image two filamentous protein components of the intracellular cytoskeleton: microtubules (MT) and intermediate filaments (IF). MT are 25 nm diameter cylinders comprised of 13 “protofilaments” which are linear chains of 8 nm X 4 nm X 4 nm “dimer” subunits. More variable than MT, IF are 10 nm diameter coils comprised of from 4 to 8 “subfilaments” which are chains of “tetramer” subunits. MT were isolated from porcine brain and IF from cell culture by standard techniques. Preparation/stabilization factors included magnesium buffer, 0.8 molar glycerol, and 0.1% glutaraldehyde. Samples were scanned on graphite in a Nanoscope I or II STM (Digital Instruments, Santa Barbara, California). STM images of MT demonstrated flattened 25 nm diameter structures composed of 4 nm wide protofilaments. Processed inverted images showed rows of 8 nm X 4 nm subunits. STM images of IF showed flattened, parallel 10 nm filaments comprised of coiled chains of 6–12 nm tetramer subunits. Thus STM demonstrated internal structure of two different classes of cytoskeletal proteins. STM and related techniques have an important future in biomolecular studies.

Original languageEnglish (US)
Pages (from-to)687-691
Number of pages5
JournalJournal of Vacuum Science and Technology A: Vacuum, Surfaces and Films
Volume8
Issue number1
DOIs
StatePublished - Jan 1990

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films

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