Scanning two-photon microscopy with upconverting lanthanide nanoparticles via Richardson-Lucy deconvolution

Christian F. Gainer, Urs Utzinger, Marek Romanowski

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The use of upconverting lanthanide nanoparticles in fast-scanning microscopy is hindered by a long luminescence decay time, which greatly blurs images acquired in a nondescanned mode. We demonstrate herein an image processing method based on Richardson-Lucy deconvolution that mitigates the detrimental effects of their luminescence lifetime. This technique generates images with lateral resolution on par with the system's performance, ∼1.2 μm, while maintaining an axial resolution of 5 μm or better at a scan rate comparable with traditional two-photon microscopy. Remarkably, this can be accomplished with near infrared excitation power densities of 850 W/cm 2, several orders of magnitude below those used in two-photon imaging with molecular fluorophores. By way of illustration, we introduce the use of lipids to coat and functionalize these nanoparticles, rendering them water dispersible and readily conjugated to biologically relevant ligands, in this case epidermal growth factor receptor antibody. This deconvolution technique combined with the functionalized nanoparticles will enable three-dimensional functional tissue imaging at exceptionally low excitation power densities.

Original languageEnglish (US)
Article number076003
JournalJournal of Biomedical Optics
Volume17
Issue number7
DOIs
StatePublished - Jul 2012

Fingerprint

Lanthanoid Series Elements
Deconvolution
Rare earth elements
Microscopic examination
Photons
Nanoparticles
microscopy
Scanning
nanoparticles
scanning
Luminescence
radiant flux density
photons
luminescence
Imaging techniques
Fluorophores
antibodies
Epidermal Growth Factor Receptor
Antibodies
Lipids

Keywords

  • Lanthanide
  • Nanoparticle
  • Optical sectioning
  • Two-photon microscopy
  • Upconversion

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Scanning two-photon microscopy with upconverting lanthanide nanoparticles via Richardson-Lucy deconvolution. / Gainer, Christian F.; Utzinger, Urs; Romanowski, Marek.

In: Journal of Biomedical Optics, Vol. 17, No. 7, 076003, 07.2012.

Research output: Contribution to journalArticle

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