Scatter sensitive microscopic techniques to identify contrasting mucosal structures in ultrahigh-resolution optical coherence tomograms of mouse colon

Alexandre R. Tumlinson, Lida P. Hariri, Wolfgang Drexler, Jennifer K. Barton

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Optical coherence tomography, optical coherence microscopy, reflectance confocal microscopy, and darkfield microscopy all derive contrast from the intensity of endogenous tissue scatter. We have imaged excised mouse colon tissue with these complimentary technologies to make conclusions about structural origins of scatter in the mouse colonic mucosa observed with endoscopic OCT. We find hyperintense scattering both from the cytoplasm of epithelial cells and from the boundary between epithelia and the lamina propria. We find almost no scatter from the portion of epithelial cells containing the nucleus. These observations substantiate explanations for the appearance of colonic crypts and the luminal surface..

Original languageEnglish (US)
Title of host publicationEndoscopic Microscopy III
DOIs
StatePublished - Apr 21 2008
EventEndoscopic Microscopy III - San Jose, CA, United States
Duration: Jan 20 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6851
ISSN (Print)1605-7422

Other

OtherEndoscopic Microscopy III
CountryUnited States
CitySan Jose, CA
Period1/20/081/21/08

Keywords

  • Confocal
  • Contrast
  • Darkfield
  • Epithelia
  • Gastrointestinal
  • Microscopy
  • OCM
  • Optical coherence tomography OCT
  • Unstained

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Tumlinson, A. R., Hariri, L. P., Drexler, W., & Barton, J. K. (2008). Scatter sensitive microscopic techniques to identify contrasting mucosal structures in ultrahigh-resolution optical coherence tomograms of mouse colon. In Endoscopic Microscopy III [68510A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6851). https://doi.org/10.1117/12.763968