SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2

Daniel W. Buster, Scott G. Daniel, Huy Q. Nguyen, Sarah L. Windler, Lara C. Skwarek, Maureen Peterson, Meredith Roberts, Joy H. Meserve, Tom Hart, Joseph E. Klebba, David Bilder, Giovanni Bosco, Gregory C. Rogers

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Condensin complexes play vital roles in chromosome condensation during mitosis and meiosis. Condensin II uniquely localizes to chromatin throughout the cell cycle and, in addition to its mitotic duties, modulates chromosome organization and gene expression during interphase. Mitotic condensin activity is regulated by phosphorylation, but mechanisms that regulate condensin II during interphase are unclear. Here, we report that condensin II is inactivated when its subunit Cap-H2 is targeted for degradation by the SCFSlimb ubiquitin ligase complex and that disruption of this process dramatically changed interphase chromatin organization. Inhibition of SCFSlimb function reorganized interphase chromosomes into dense, compact domains and disrupted homologue pairing in both cultured Drosophila cells and in vivo, but these effects were rescued by condensin II inactivation. Furthermore, Cap-H2 stabilization distorted nuclear envelopes and dispersed Cid/CENP-A on interphase chromosomes. Therefore, SCFSlimb mediated down-regulation of condensin II is required to maintain proper organization and morphology of the interphase nucleus.

Original languageEnglish (US)
Pages (from-to)49-63
Number of pages15
JournalJournal of Cell Biology
Volume201
Issue number1
DOIs
StatePublished - Apr 2013

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Ligases
Ubiquitin
Interphase
Chromosomes
Chromatin
Nuclear Envelope
Meiosis
condensin complexes
Mitosis
Drosophila
Cultured Cells
Cell Cycle
Down-Regulation
Phosphorylation
Gene Expression

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2. / Buster, Daniel W.; Daniel, Scott G.; Nguyen, Huy Q.; Windler, Sarah L.; Skwarek, Lara C.; Peterson, Maureen; Roberts, Meredith; Meserve, Joy H.; Hart, Tom; Klebba, Joseph E.; Bilder, David; Bosco, Giovanni; Rogers, Gregory C.

In: Journal of Cell Biology, Vol. 201, No. 1, 04.2013, p. 49-63.

Research output: Contribution to journalArticle

Buster, DW, Daniel, SG, Nguyen, HQ, Windler, SL, Skwarek, LC, Peterson, M, Roberts, M, Meserve, JH, Hart, T, Klebba, JE, Bilder, D, Bosco, G & Rogers, GC 2013, 'SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2', Journal of Cell Biology, vol. 201, no. 1, pp. 49-63. https://doi.org/10.1083/jcb.201207183
Buster, Daniel W. ; Daniel, Scott G. ; Nguyen, Huy Q. ; Windler, Sarah L. ; Skwarek, Lara C. ; Peterson, Maureen ; Roberts, Meredith ; Meserve, Joy H. ; Hart, Tom ; Klebba, Joseph E. ; Bilder, David ; Bosco, Giovanni ; Rogers, Gregory C. / SCFSlimb ubiquitin ligase suppresses condensin II-mediated nuclear reorganization by degrading Cap-H2. In: Journal of Cell Biology. 2013 ; Vol. 201, No. 1. pp. 49-63.
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