Screening for antibodies associated with halothane hepatitis

K. L. Hastings, C. Thomas, A. K. Hubbard, A Jay Gandolfi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The diagnosis of halothane hepatitis (HH) may be assisted by detection of antibodies reacting to trifluoroacetylated proteins (anti- TFA antibodies). An enzyme-linked immunosorbent assay (ELISA) utilizing trifluoroacetylated rabbit serum albumin (TFA-RSA) as antigen detected anti-TFA antibodies in 67% of sera from patients for whom a clinical diagnosis of HH was made. Anti-TFA antibodies were detected in 33% of sera when using an EL ISA with liver microsomal protein from halothane-treated rabbits as antigen. Absorption of the sera with untreated rabbit liver microsomal protein before using the microsomal protein ELISA resulted in detection of anti-TFA antibodies in 42% of sera. Using the presumptive hapten N-e-trifluoroacetyl-l-lysine to block antibody binding in an ELISA resulted in positive detection in 50% of sera: the results did not always agree with the other ELISA methods. The TFA-RSA ELISA was the most sensitive method and, combined with the TFA-lysine blocking ELISA, resulted in 92% of sera from HH patients testing positive for HH-associated antibodies.

Original languageEnglish (US)
Pages (from-to)722-728
Number of pages7
JournalBritish Journal of Anaesthesia
Volume67
Issue number6
DOIs
StatePublished - Dec 1991

Fingerprint

Halothane
Enzyme-linked Immunosorbent Assay
halothane
hepatitis
antibody detection
Antibody
Antibodies
Screening
Enzyme-Linked Immunosorbent Assay
Assays
enzyme-linked immunosorbent assay
Enzymes
antibodies
Rabbit
Anti-Idiotypic Antibodies
Serum
rabbits
Rabbits
Proteins
Protein

Keywords

  • Anaesthetics, volatile: halothane.
  • Complications: halothane hepatitis.
  • Immune response: anti-TFA antibodies.

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Mathematics(all)
  • Statistics and Probability
  • Agricultural and Biological Sciences (miscellaneous)
  • Anesthesiology and Pain Medicine

Cite this

Screening for antibodies associated with halothane hepatitis. / Hastings, K. L.; Thomas, C.; Hubbard, A. K.; Gandolfi, A Jay.

In: British Journal of Anaesthesia, Vol. 67, No. 6, 12.1991, p. 722-728.

Research output: Contribution to journalArticle

Hastings, K. L. ; Thomas, C. ; Hubbard, A. K. ; Gandolfi, A Jay. / Screening for antibodies associated with halothane hepatitis. In: British Journal of Anaesthesia. 1991 ; Vol. 67, No. 6. pp. 722-728.
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