Screening for mRNA expression using the polymerase chain reaction

Ivana Fantozzi, Carmelle V. Remillard, Mehran Mandegar, Jason Yuan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Polymerase chain reaction (PCR) is an in vitro technique for amplifying a target DNA sequence in a short time. This is because both strands of DNA are copied simultaneously during PCR; therefore, there is an exponential increase in the number of copies of the target DNA fragment or gene with each cycle (1,2). Three fundamental aspects make PCR such a powerful tool in determining the qualitative expression of target genes: 1. PCR is less expensive, less time-consuming, and simpler than previous DNA (and RNA) duplication techniques, making it possible for anyone, even one with no training in molecular biology, to do genetic and molecular biological research.

Original languageEnglish (US)
Title of host publicationIon Channels in the Pulmonary Vasculature
PublisherCRC Press
Pages711-732
Number of pages22
ISBN (Electronic)9780849350375
ISBN (Print)0824759680, 9780824759681
StatePublished - Jan 1 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Medicine(all)

Cite this

Fantozzi, I., Remillard, C. V., Mandegar, M., & Yuan, J. (2005). Screening for mRNA expression using the polymerase chain reaction. In Ion Channels in the Pulmonary Vasculature (pp. 711-732). CRC Press.