Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage φX174

B. A. Fane, S. Shien, M. Hayashi

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Abstract

This report describes the isolation and characterization of second-site suppressors of a cold-sensitive (cs) external scaffolding protein, gpD, of bacteriophage φX174. Seven genetically distinct suppressors were isolated. Six of them are located in gene F which encodes the major coat protein of the virus. The seventh is located in gene J which encodes the DNA-binding protein. A subset of the suppressors are trans-acting. These second-site suppressors do not exhibit allele specificity; they are able to suppress defects associated with a csD protein for which they were not selected. The initial characterization of the second-site suppressors and their locations within the major coat protein suggest that the mechanism of suppression may involve both structural and stoichiometric phenomena.

Original languageEnglish (US)
Pages (from-to)1003-1011
Number of pages9
JournalGenetics
Volume134
Issue number4
StatePublished - Jan 1 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

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