Second-site suppressors of a cold-sensitive prohead accessory protein of bacteriophage ΦX174

B. A. Fane, M. Hayashi

Research output: Contribution to journalArticle

52 Scopus citations


This study describes the isolation of second-site suppressors which correct for the defects associated with cold-sensitive (cs) prohead accessory proteins of bacteriophage ΦX174. Five phenotypically different suppressors were isolated. Three of these suppressors confer novel temperature-sensitive (ts) phenotypes. They were unable to complement a ts mutation in gene F which encodes the major coat protein of the phage. All five suppressor mutations confer nucleotide changes in the gene F DNA sequence. These changes define four amino acid sites in the gene F protein. Three suppressor mutations placed into an otherwise wild-type background display a cold resistant phenotype in liquid culture infections when compared to a wild-type ΦX174 control.

Original languageEnglish (US)
Pages (from-to)663-671
Number of pages9
Issue number4
StatePublished - Oct 28 1991
Externally publishedYes


ASJC Scopus subject areas

  • Genetics

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